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Lysophosphatidylcholine Activates Adipocyte Glucose Uptake and Lowers Blood Glucose Levels in Murine Models of Diabetes

Cited 64 time in Web of Science Cited 65 time in Scopus
Authors
Yea, Kyungmoo; Kim, Jaeyoon; Yoon, Jong Hyuk; Kwon, Taewan; Lee, Byoung Dae; Lee, Seung Jae; Lee, Taehoon G.; Park, Ho Seon; Ohba, Motoi; Ryu, Sung Ho; Suh, Pann-Ghill; Park, Kyong Soo; Baek, Moon-Chang; Kim, Jong-In; Lee, Hae-Jeong; Kim, Jong Hyun
Issue Date
2009-12-04
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Citation
JOURNAL OF BIOLOGICAL CHEMISTRY; Vol.284 49; 33833-33840
Abstract
Glucose homeostasis is maintained by the orchestration of peripheral glucose utilization and hepatic glucose production, mainly by insulin. In this study, we found by utilizing a combined parallel chromatography mass profiling approach that lysophosphatidylcholine (LPC) regulates glucose levels. LPC was found to stimulate glucose uptake in 3T3-L1 adipocytes dose- and time-dependently, and this activity was found to be sensitive to variations in acyl chain lengths and to polar head group types in LPC. Treatment with LPC resulted in a significant increase in the level of GLUT4 at the plasma membranes of 3T3-L1 adipocytes. Moreover, LPC did not affect IRS-1 and AKT2 phosphorylations, and LPC-induced glucose uptake was not influenced by pretreatment with the PI 3-kinase inhibitor LY294002. However, glucose uptake stimulation by LPC was abrogated both by rottlerin (a protein kinase C delta inhibitor) and by the adenoviral expression of dominant negative protein kinase C delta. In line with its determined cellular functions, LPC was found to lower blood glucose levels in normal mice. Furthermore, LPC improved blood glucose levels in mouse models of type 1 and 2 diabetes. These results suggest that an understanding of the mode of action of LPC may provide a new perspective of glucose homeostasis.
ISSN
0021-9258
Language
English
URI
http://hdl.handle.net/10371/76252
DOI
https://doi.org/10.1074/jbc.M109.024869
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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