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Leukemia-specific siRNA delivery by immunonanoplexes consisting of anti-JL1 minibody conjugated to oligo-9 Arg-peptides

Cited 16 time in Web of Science Cited 16 time in Scopus
Authors
Lee, Yeon Kyung; Kim, Keun Sik; Kim, Jung Seok; Baek, Jin Ee; Jeong, Hwa Yeon; Jung, Kyeong Cheon; Park, Yong Serk; Song, Hyung Geun; Yoon, Sang Soon; Park, Sang Il
Issue Date
2010-05
Publisher
KOREAN SOC MOLECULAR & CELLULAR BIOLOGY
Citation
MOLECULES AND CELLS; Vol.29 5; 457-462
Keywords
9-arginine peptidesiRNAleukemia therapyimmunonanoplexanti-JL1 minibody
Abstract
Targeted mRNA degradation by short interfering RNAs (siRNAs) offers a great potential to treat cancers. siRNA therapeutics for leukemias are, however, hindered by poor intracellular uptake, limited blood stability and nonspecific delivery. To solve these problems, we developed an anti-JL1 immunonanoplex (antibody-coupled nanocomplex) for siRNA delivery using anti-JL1 minibody (leukemia cell-specific minibody) conjugated to oligo-9-Arg peptide (9R) for effective siRNA delivery to leukemic cells. The anti-JL1 immunonanoplexes were able to deliver siRNA specifically to leukemic cells (CEM and Jurkat), but not to control cancer cells (H9). According to FACS and confocal microscopic analysis, siRNAs delivered by immunonanoplex particles were rapidly taken up by the JL1-positive cancer cells in 2 h. Furthermore, we showed that the anti-JL1 immunonanoplexes were effectively targeted to JL1-positive cells (CEM) inoculated in the mouse bone marrow. These results suggest that the anti-JL1 immunonanoplex is a powerful siRNA delivery system for human leukemia therapies.
ISSN
1016-8478
Language
English
URI
http://hdl.handle.net/10371/76675
DOI
https://doi.org/10.1007/s10059-010-0056-5
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College of Medicine/School of Medicine (의과대학/대학원)Pathology (병리학전공)Journal Papers (저널논문_병리학전공)
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