Browse

Glutamine attenuates acute lung injury by inhibition of high mobility group box protein-1 expression during sepsis

Cited 36 time in Web of Science Cited 39 time in Scopus
Authors
Kwon, Woon Yong; Suh, Gil Joon; Kim, Kyung Su; Jo, You Hwan; Kim, Kyuseok; Jung, Sung Koo; Lee, Jae Hyuk
Issue Date
2010-03-28
Publisher
CAMBRIDGE UNIV PRESS
Citation
BRITISH JOURNAL OF NUTRITION; Vol.103 6; 890-898
Keywords
GlutamineHigh mobility group box protein-1Lung injurySepsis
Abstract
Heat shock protein 70 (HSP70) is reported as the main factor responsible for the beneficial effects of glutamine (GLN) and as a negative regulator of high mobility group box protein-1 (HMGB-1) expression. Our aim was to determine whether GLN attenuates acute lung injury (ALI) by the inhibition of HMGB-1 expression during sepsis. Male Sprague Dawley rats were subjected to caecal ligation and puncture (CLP) to induce sepsis. GLN or saline was administered through tail vein 1 h after CLP. Then, quercetin (Q), an inhibitor of HSP70, was utilised to assess the role of the enhanced HSP70. We observed the survival of the subjects. At 24h post-CLP, we measured lung HSP70, phosphorylated heat shock factor-1 (HSF-1-p) and HMGB-1 expressions, NF-kappa B DNA-binding activity and ALI occurrence. We also measured serum HSP70, IL-6 and HMGB-1 concentrations. GLN improved survival during sepsis. In GLN-treated rats, lung HSP70 and HSF-1-p expressions were enhanced, lung HMGB-1 expression and NF-kappa B DNA-binding activity were suppressed, and ALI was attenuated. Furthermore, in GLN-administered rats, serum HSP70 concentration was higher, and serum IL-6 and HMGB-1 concentrations were lower than those in non-treated rats. Q inhibited the enhancement of HSP70 and HSF-1-p expressions and abrogated the GLN-mediated benefits. In conclusion, GLN attenuated ALI and improved survival by the inhibition of HMGB-1 expression during sepsis in rats. These benefits were associated with the enhancement of HSP70 expression by GLN.
ISSN
0007-1145
Language
English
URI
http://hdl.handle.net/10371/78048
DOI
https://doi.org/10.1017/S0007114509992509
Files in This Item:
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Emergency Medicine (응급의학전공)Journal Papers (저널논문_응급의학전공)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse