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Neutrophil Migration in Opposing Chemoattractant Gradients Using Microfluidic Chemotaxis Devices

Cited 79 time in Web of Science Cited 81 time in Scopus
Authors
Lin, Francis; Nguyen, Connie Minh-Canh; Wang, Shur-Jen; Saadi, Wajeeh; Gross, Steven P.; Jeon, Noo Li
Issue Date
2005-04
Publisher
Springer Verlag
Citation
Ann. Biomed. Eng. 33:475-482, 2005
Keywords
ChemotaxisGradientHierarchyMicrofluidicMigration
Abstract
Neutrophils migrating in tissue respond to complex overlapping signals generated by a variety of chemotactic factors (CFs). Previous studies suggested a hierarchy between bacteriaderived CFs and host-derived CFs but could not differentiate neutrophil
response to potentially equal host-derived CFs (IL-8 and LTB4). This paper reports neutrophil migration in conflicting gradients
of IL-8 and LTB4 using a microfluidic chemotaxis device that can generate stable and well-defined gradients. We quantitatively
characterized the movement of cells from time-lapse images. Neutrophils migrate more efficiently toward single IL-8 gradients than single LTB4 gradients as measured by the effective chemotactic index (ECI). In opposing gradients of IL-8 and LTB4,
neutrophils show obvious chemotaxis toward a distant gradient, consistent with previous reports. When an opposing gradient of
LTB4 is present, neutrophils show less effective chemotaxis toward IL-8 than when they are in a gradient of IL-8 alone. In
contrast, the chemotactic response of neutrophils to LTB4 is not reduced in opposing gradients as compared to that in a single LTB4 gradient. These results indicate that the presence of one host-derived CF modifies the response of neutrophils to a second CF suggesting a subtle hierarchy between them.
ISSN
0090-6964 (print)
1573-9686 (online)
Language
English
URI
http://hdl.handle.net/10371/7988
DOI
https://doi.org/10.1007/s10439-005-2503-6
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Mechanical Aerospace Engineering (기계항공공학부)Journal Papers (저널논문_기계항공공학부)
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