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Isolation and characterization of equine amniotic membrane-derived mesenchymal stem cells

Cited 16 time in Web of Science Cited 18 time in Scopus
Authors
Seo, Min-Soo; Park, Sang-Bum; Kim, Hyung-Sik; Kang, Jun-gu; Chae, Joon-Seok; Kang, Kyung-Sun
Issue Date
2013-06
Publisher
The Korean Society of Veterinary Science
대한수의학회
Citation
Journal of Veterinary Science Vol.14 No.2, pp. 151-159
Keywords
의약학amnionamniotic membraneequineisolationmesenchymal stem cells
Abstract
Recent studies have shown that mesenchymal stem cells (MSCs) are able to differentiate into multi-lineage cells such as adipocytes, chondroblasts, and osteoblasts. Amniotic membrane from whole placenta is a good source of stem cells in humans. This membrane can potentially be used for wound healing and corneal surface reconstruction. Moreover, it can be easily obtained after delivery and is usually discarded as classified waste. In the present study, we successfully isolated and characterized equine amniotic membrane-derived mesenchymal stem cells (eAM-MSCs) that were cultured and maintained in low glucose Dulbecco’s modified Eagle’s medium. The proliferation of eAM-MSCs was measured based on the cumulative population doubling level (CPDL). Immunophenotyping of eAM-MSCs by flow cytometry showed that the major population was of mesenchymal origin. To confirm differentiation potential, a multi-lineage differentiation assay was conducted. We found that under appropriate conditions, eAM-MSCs are capable of multi-lineage differentiation. Our results indicated that eAM-MSCs may be a good source of stem cells, making them potentially useful for veterinary regenerative medicine and cell-based therapy.
ISSN
1229-845X (print)
1976-555X (online)
Language
English
URI
http://hdl.handle.net/10371/83362
DOI
https://doi.org/10.4142/jvs.2013.14.2.151
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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