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A recombinant human G-CSF/GM-CSF fusion protein from E-coli showing colony stimulating activity on human bone marrow cells

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dc.contributor.authorLee, Ah Young-
dc.contributor.authorChung, Hye Kyung-
dc.contributor.authorBae, Eun Kyong-
dc.contributor.authorHwang, Jung Suk-
dc.contributor.authorSung, Bong Whan-
dc.contributor.authorCho, Chung Won-
dc.contributor.authorKim, Jin-Kyoo-
dc.contributor.authorLee, Kyung-
dc.contributor.authorHan, Jae Yong-
dc.contributor.authorLee, Choon-Taek-
dc.contributor.authorYoun, Hyun Joo-
dc.date.accessioned2017-01-23T07:49:53Z-
dc.date.available2017-01-23T07:49:53Z-
dc.date.issued2003-
dc.identifier.citationBiotechnology Letters, vol.25 no.3, pp. 205-211ko_KR
dc.identifier.issn0141-5492-
dc.identifier.urihttps://hdl.handle.net/10371/100194-
dc.description.abstractGranulocyte-Macrophage colony stimulating factor (GM-CSF) and Granulocyte colony stimulating factor (G-CSF) are cytokines involved in the differentiation of bone marrow progenitor cells into myeloid cells. They also activate mature myeloid cells to mediate a variety of antimicrobial activities and inflammatory responses. Recombinant GM-CSF and G-CSF proteins have been used to treat various diseases including cancer and hematopoietic diseases and to isolate peripheral blood progenitor cells for bone marrow transplantation. A plasmid construct expressing recombinant human G-CSF/GM-CSF fusion protein has now been prepared by linking the human G-CSF and GMCSF coding regions and the recombinant fusion protein has been successfully expressed in E. coli. The recombinant human G-CSF/GM-CSF fusion protein was extracted and purified from the cellular inclusion and refolded into the biologically active form to show colony stimulating activity. The recombinant fusion protein exhibited colony stimulating activity on human bone marrow cell cultures, indicating that the linkage of GM-CSF and G-CSF by a linker peptide may not interrupt activities of the cytokines in the fusion protein. The colony forming unit of the fusion protein was also higher than those of the cultures treated with the same molar numbers of the recombinant human GM-CSF and G-CSF separately, which suggests that the fusion protein presumably retains both G-CSF and GM-CSF activities.ko_KR
dc.language.isoenko_KR
dc.publisherSpringerko_KR
dc.titleA recombinant human G-CSF/GM-CSF fusion protein from E-coli showing colony stimulating activity on human bone marrow cellsko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor이아영-
dc.contributor.AlternativeAuthor정혜경-
dc.contributor.AlternativeAuthor배은경-
dc.contributor.AlternativeAuthor황정석-
dc.contributor.AlternativeAuthor성봉환-
dc.contributor.AlternativeAuthor조정원-
dc.contributor.AlternativeAuthor김진규-
dc.contributor.AlternativeAuthor이경-
dc.contributor.AlternativeAuthor한재용-
dc.contributor.AlternativeAuthor이춘택-
dc.contributor.AlternativeAuthor윤현주-
dc.identifier.doi10.1023/A:1022346800375-
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