Publications
Detailed Information
Improved embryo development with decreased apoptosis in blastomeres after the treatment of cloned bovine embryos with beta-mercaptoethanol and hemoglobin
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Eul Soon | - |
dc.contributor.author | Hwang, Woo Suk | - |
dc.contributor.author | Kang, Sung Keun | - |
dc.contributor.author | Lee, Byeong Chun | - |
dc.contributor.author | Han, Jae Yong | - |
dc.contributor.author | Lim, Jeong Mook | - |
dc.date.accessioned | 2017-01-23T07:53:05Z | - |
dc.date.available | 2017-01-23T07:53:05Z | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | Molecular Reproduction and Development, vol.67 no.2, pp. 200-206 | ko_KR |
dc.identifier.issn | 1040-452X | - |
dc.identifier.uri | https://hdl.handle.net/10371/100200 | - |
dc.description.abstract | In preliminary experiments, the treatment of donor somatic cells with b-mercaptoethanol (ME) or hemoglobin (Hb) improved in vitrodevelopment of bovine cloned embryos. This study was subsequently evaluated whether the exposure to Hb and/or ME during in vitro-maturation or embryo culture could further promote the development of embryos cloned with ME-treated donor cells. A prospective, randomized study was conducted and, embryo development, cell number, and apoptosis in blastocysts were monitored. A significant (P<0.05) effect was found after the combined treatment of cloned embryos with Hb (1 mg/ml) and ME (10 mM); the development of morulae (53 vs. 35%) was greatly improved, which resulted in enhanced blastocyst formation (38%). However, cell number and apoptosis in blastocysts were predominantly affected by ME rather than Hb; a significant increase in total cell number of blastomeres (142–154 vs. 123 cells/embryo), inner cell mass (ICM) (39–41 vs. 27), and trophectoderm (TE) (103–114 vs. 98), and the ratio of ICM to TE cell number (0.26–0.27 vs. 0.22) was found. Also, the apoptosis index indicating the ratio of apoptotic cell to normal blastomere number was greatly reduced after ME treatments (0.85 vs. 0.056–0.069). When embryos cloned with ME-treated cells were cultured in HbþME-containing medium, any of the treatments to recipient oocytes before enucleation did not further promote the development. In conclusion, combined treatment of cloned embryos with HbþME not only improved in vitro-development but also decreased blastomere apoptosis. The use of ME-treated donor cells and the culture of cloned embryos in HbþMEcontaining medium yielded the optimal results for promoting the production of blastocysts with improved quality. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | Wiley | ko_KR |
dc.subject | somatic cell nuclear transfer | ko_KR |
dc.subject | apoptosis | ko_KR |
dc.subject | preimplantation development | ko_KR |
dc.subject | hemoglobin | ko_KR |
dc.subject | b-mercaptoethanol | ko_KR |
dc.title | Improved embryo development with decreased apoptosis in blastomeres after the treatment of cloned bovine embryos with beta-mercaptoethanol and hemoglobin | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 박을순 | - |
dc.contributor.AlternativeAuthor | 황우석 | - |
dc.contributor.AlternativeAuthor | 강성근 | - |
dc.contributor.AlternativeAuthor | 이병준 | - |
dc.contributor.AlternativeAuthor | 한재용 | - |
dc.contributor.AlternativeAuthor | 임정묵 | - |
dc.identifier.doi | 10.1002/mrd.10392 | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.