S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Food and Animal Biotechnology (식품·동물생명공학부) Journal Papers (저널논문_식품·동물생명공학부)
Incidence of apoptosis in clone embryos and improved development by the treatment of donor somatic cells with putative apoptosis inhibitors
- Park, E.S.; Hwang, W.S.; Jang, G.; Cho, J.K.; Kang, S.K.; Lee, B.C.; Han, Jae Yong; Lim, J.M.
- Issue Date
- Molecular Reproduction and Development, vol.68 no.1, pp. 65-71
- This study was conducted to promote in vitro-development of clone embryos by the treatment of donor somatic cells with hemoglobin (Hb) and/or b-mercaptoethanol (ME), based on the analysis of apoptosis after somatic cell nuclear transfer (SCNT). Prospective, randomized study was conducted and, in vitro-matured bovine oocytes and fetal fibroblasts were provided for SCNT. In the first series of experiment, embryo apoptosis after SCNT was monitored by a terminal deoxynucleotidyl transferase-mediated d-UTP nick end-labeling assay. As results, apoptosis occurred more (P<0.05) frequently after SCNT than after in vitro-fertilization (IVF) of control treatment. Subsequently, donor somatic cells treated with Hb (1 mg/ml) and/or ME (10 mM) were provided for SCNT. Either Hb or ME greatly reduced apoptosis (0.0830.006 vs. 0.058–0.0680.005), while combined treatment did not. ME was more promotive than Hb; significant increases were found in morula compaction (86%), cell numbers of blastocyst (131.31.3 cells/blastocyst), and inner cell mass (31.90.8 cells/blastocyst) cell, and the ratio of inner cell mass to trophectodermal cell numbers (0.240.01). In conclusion, the treatment of donor somatic cells with ME or Hb could reduce apoptosis after SCNT, resulting improved preimplantation development.
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