Publications
Detailed Information
Enriched gonadal migration of donor-derived gonadal primordial germ cells by immunomagnetic cell sorting in birds
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Jin Nam | - |
dc.contributor.author | Kim, Mi A | - |
dc.contributor.author | Park, Tae Sub | - |
dc.contributor.author | Kim, Duk Kyung | - |
dc.contributor.author | Park, Hyun Jeong | - |
dc.contributor.author | Ono, Tamao | - |
dc.contributor.author | Lim, Jeong Mook | - |
dc.contributor.author | Han, Jae Yong | - |
dc.date.accessioned | 2017-01-23T07:54:09Z | - |
dc.date.available | 2017-01-23T07:54:09Z | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | Molecular Reproduction and Development, vol.68 no.1, pp. 81-87 | ko_KR |
dc.identifier.issn | 1040-452X | - |
dc.identifier.uri | https://hdl.handle.net/10371/100202 | - |
dc.description.abstract | This study was conducted to evaluate whether immunomagnetic treatment could improve the retrieval and migration capacity of avian gonadal primordial germ cells (gPGCs) collected from gonads in 5.5-day-old chick and 5-day-old quail embryos, respectively. Collected gPGCs were loaded into a magnetic-activated cell sorter (MACS) after being conjugated with specific gPGC antibodies and either MACS-treated or non-treated cells in each species were subsequently transferred to the recipient embryos. MACS treatment significantly (P<0.05) increased the population ratio of gPGCs in gonadal cells retrieved (0.74 to 33.4% in the chicken and 2.68 to 45.1% in the quail). This was due to decreased number of nongPGCs in total cell population. MACS treatment further enhanced gonadal migration of gPGCs transferred in both species (10% vs. 80–85% in the chicken and 10–15% vs. 70–80% in the quail). Increase in the number of microinjected cells up to 600 cells/embryo did not eliminate such promoting effect. In conclusion, MACS treatment greatly increased the population ratio of avian gPGCs in gonadal cells, resulting improved gonadal migration in recipient embryos. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | Wiley | ko_KR |
dc.subject | gonadal primordial germ cell (gPGC) | ko_KR |
dc.subject | immunomagnetic treatment | ko_KR |
dc.subject | chicken | ko_KR |
dc.subject | quail | ko_KR |
dc.subject | gonadal migration | ko_KR |
dc.title | Enriched gonadal migration of donor-derived gonadal primordial germ cells by immunomagnetic cell sorting in birds | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 김진남 | - |
dc.contributor.AlternativeAuthor | 김미아 | - |
dc.contributor.AlternativeAuthor | 박태섭 | - |
dc.contributor.AlternativeAuthor | 김덕경 | - |
dc.contributor.AlternativeAuthor | 박현정 | - |
dc.contributor.AlternativeAuthor | 임정묵 | - |
dc.contributor.AlternativeAuthor | 한재용 | - |
dc.identifier.doi | 10.1002/mrd.20051 | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.