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Development of a nonmechanical enucleation method using x-ray irradiation in somatic cell nuclear transfer

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Authors
Kim, Tae M.; Hwang, Woo S.; Shin, Ji H.; Park, Hyun J.; Han, Jae Yong; Lim, Jeong M.
Issue Date
2004
Publisher
Elsevier
Citation
Fertility and Sterility, vol.82 no.4, pp. 963-965
Abstract
Irradiation of in-vitro-matured bovine oocytes with x-rays of different durations was performed to develop an
alternative to conventional mechanical enucleation methods in somatic cell nuclear transfer. No significant
difference in embryo development to the blastocyst stage was detected between nonmechanical and mechanical
methods, and cytologic analyses of karyotype and microtubule formation showed the potential availability
of x-ray irradiation.
ISSN
0015-0282
Language
English
URI
https://hdl.handle.net/10371/100204
DOI
https://doi.org/10.1016/j.fertnstert.2004. 03.047
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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