S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Food and Animal Biotechnology (식품·동물생명공학부) Journal Papers (저널논문_식품·동물생명공학부)
Serum replacement with a growth factor-free synthetic substance in culture medium contributes to effective establishment of mouse embryonic stem cells of various origins
- Lee, Seung Tae; Oh, Se Woong; Kim, Dae Yong; Han, Jae Yong; Moon, Shin Yong; Lim, Jeong Mook
- Issue Date
- Fertility and Sterility, vol.86 no.4, pp. 1137-1145
- Objective: To evaluate whether serum replacement with growth factor–free synthetic substances contributed to
the effective establishment of embryonic stem (ES) cells.
Design: Randomized, prospective model study.
Setting: Gamete and stem cell biotechnology laboratory at Seoul National University in Korea.
Animal(s): F1 (C57BL6 DBA2) mice.
Intervention(s): Blastocysts of different origins were cultured in serum-replaced media.
Main Outcome Measure(s): Embryonic stem cell establishment.
Result(s): Eight batches of ES cells were established from colony-forming inner cell mass cells after the
replacement of fetal bovine serum (FBS) with synthetic knockout serum replacement (KSR) in mkDMEM. The
established cells were positive for ES cell markers and formed both embryoid bodies in vitro and teratomas in
vivo, but the established cell batches and control (transformed) ES cells responded differently to the culture
media. Higher levels of cell viability were detected after the replacement with the 75:25 FBS–KSR mixture than
with any other mixtures, and a gradual decrease in viability was detected as the KSR volume ratio was increased.
The 75:25 FBS–KSR mixture-containing medium supported ES cell establishment of outbred ICR, F1, and F2 of
C57BL6/DBA2; F1 parthenogenetic and ES cell–complemented tetraploid blastocysts; and single ES-cell
Conclusion(s): A serum-replaced medium could be used for effective ES-cell establishment of various origins.
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