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Generation of transgenic quail through germ cell-mediated germline transmission
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shin, Sang Su | - |
dc.contributor.author | Kim, Tae Min | - |
dc.contributor.author | Kim, Sun Young | - |
dc.contributor.author | Kim, Tae Wan | - |
dc.contributor.author | Seo, Hee Won | - |
dc.contributor.author | Lee, Seul Ki | - |
dc.contributor.author | Kwon, Se Chang | - |
dc.contributor.author | Lee, Gwan Sun | - |
dc.contributor.author | Kim, Heebal | - |
dc.contributor.author | Lim, Jeong Mook | - |
dc.contributor.author | Han, Jae Yong | - |
dc.date.accessioned | 2017-01-25T09:15:25Z | - |
dc.date.available | 2017-01-25T09:15:25Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | FASEB Journal, vol.22 no.7, pp. 2435-2444 | ko_KR |
dc.identifier.issn | 0892-6638 | - |
dc.identifier.uri | https://hdl.handle.net/10371/100257 | - |
dc.description.abstract | Here, we describe the production of transgenic quail via a germline transmission system using postmigratory gonadal primordial germ cells (gPGCs). gPGCs retrieved from the embryonic gonads of 5-day-old birds were transduced with a lentiviral vector and subsequently transferred into recipient embryos. Testcross and genetic analyses revealed that among three germline chimeric G0 quail, one male produced transgenic offspring; of 310 hatchlings from the transgenic germline chimera, 24 were identified as donor-derived offspring, and 6 were transgenic (6/310, 1.9%). Conventional transgenesis using stage X blastodermal embryos was also conducted, but the efficiency of transgenesis was similar between the two systems (<1.6 vs. 1.9% for the conventional and gPGC-mediated systems, respectively). However, substantial advantages can be gained from gPGC-mediated method in that it enables an induced germline modification, whereas direct retroviral transfer to stage X embryos causes mosaic integration. The use of gonadal PGCs for transgenesis may lead to the production of bioreactors. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | Federation of American Society of Experimental Biology | ko_KR |
dc.subject | primordial germ cells | ko_KR |
dc.subject | lentiviral vector | ko_KR |
dc.subject | avian transgenesis | ko_KR |
dc.title | Generation of transgenic quail through germ cell-mediated germline transmission | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 신상수 | - |
dc.contributor.AlternativeAuthor | 김태민 | - |
dc.contributor.AlternativeAuthor | 김선영 | - |
dc.contributor.AlternativeAuthor | 김태완 | - |
dc.contributor.AlternativeAuthor | 서희원 | - |
dc.contributor.AlternativeAuthor | 이슬기 | - |
dc.contributor.AlternativeAuthor | 권세창 | - |
dc.contributor.AlternativeAuthor | 이관순 | - |
dc.contributor.AlternativeAuthor | 김희발 | - |
dc.identifier.doi | 10.1096/fj.07-101485 | - |
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