S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Food and Animal Biotechnology (식품·동물생명공학부) Journal Papers (저널논문_식품·동물생명공학부)
Sexually dimorphic gene expression in the chick brain before gonadal differentiation
- Lee, S.I.; Lee, W.K.; Shin, J.H.; Han, B. Ku; Moon, S.; Cho, S.; Park, T.; Kim, H.; Han, Jae Yong
- Issue Date
- Oxford University Press
- Poultry Science Journal, vol.88 no.5, pp. 1003-1015
- chicken; sexual brain differentiation; differentially expressed gene; quantitative real-time polymerase chain reaction
- Biological bases for sexual differences in the brain exist in a wide range of vertebrate species, including chickens. Recently, the dogma of hormonal dependence for the sexual differentiation of the brain has been challenged. We examined whether sexually dimorphic gene expression in the brain precedes gonadal differentiation. Using the Affymetrix GeneChip Chicken Genome Array, we identified 42 female- and 167 male-enhanced genes that were differentially expressed in sex-specific brains from stage 29 chicken embryos. To confirm the efficacy of the microarray, and to investigate the stage-specific expression patterns of the identified genes, we used quantitative real-time PCR analysis. Our real-time PCR results for the differentially expressed genes agreed well with our microarray results. Thus, we postulate that these genes have potential roles in the sexual differentiation of neural function and development in chickens.
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