Publications

Detailed Information

The reversible developmental unipotency of germ cells in chicken

DC Field Value Language
dc.contributor.authorJung, Jin Gyoung-
dc.contributor.authorLee, Young Mok-
dc.contributor.authorKim, Jin Nam-
dc.contributor.authorKim, Tae Min-
dc.contributor.authorShin, Ji Hye-
dc.contributor.authorKim, Tae Hyun-
dc.contributor.authorLim, Jeong Mook-
dc.contributor.authorHan, Jae Yong-
dc.date.accessioned2017-01-31T04:32:50Z-
dc.date.available2017-01-31T04:32:50Z-
dc.date.issued2010-
dc.identifier.citationReproduction, vol.139 no.1, pp. 113-119ko_KR
dc.identifier.issn1470-1626-
dc.identifier.urihttps://hdl.handle.net/10371/100281-
dc.description.abstractWe recently developed bimodal germline chimera production approaches by transfer of primordial germ cells (PGCs) or embryonic germ cells (EGCs) into embryos and by transplantation of spermatogonial stem cells (SSCs) or germline stem cells (GSCs) into adult testes. This study was undertaken to investigate the reversible developmental unipotency of chicken germ cells using our established germline chimera production systems. First, we transferred freshly isolated SSCs from adult testis or in vitro cultured GSCs into stage X and stage 14–16 embryos, and we found that these transferred SSCs/GSCs could migrate to the recipient embryonic gonads. Of the 527 embryos that received SSCs or GSCs, 135 yielded hatchlings. Of 17 sexually mature males (35.3%), six were confirmed as germline chimeras through testcross analysis resulting in an average germline transmission efficiency of 1.3%. Second, PGCs/EGCs, germ cells isolated from embryonic gonads were transplanted into adult testes. The EGC transplantation induced germline transmission, whereas the PGC transplantation did not. The germline transmission efficiency was 12.5 fold higher (16.3 vs 1.3%) in EGC transplantation into testis (EGCs to adult testis) than that in SSC/GSC transfer into embryos (testicular germ cells to embryo stage). In conclusion, chicken germ cells from different developmental stages can (de)differentiate into gametes even after the germ cell developmental clock is set back or ahead. Use of germ cell reversible unipotency might improve the efficiency of germ cell-mediated germline transmission.ko_KR
dc.language.isoenko_KR
dc.publisherBioScientificako_KR
dc.titleThe reversible developmental unipotency of germ cells in chickenko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor정진경-
dc.contributor.AlternativeAuthor이영목-
dc.contributor.AlternativeAuthor김진남-
dc.contributor.AlternativeAuthor김태민-
dc.contributor.AlternativeAuthor신지혜-
dc.contributor.AlternativeAuthor김태현-
dc.contributor.AlternativeAuthor임정묵-
dc.contributor.AlternativeAuthor한재용-
dc.identifier.doi10.1530/REP-09-0265-
Appears in Collections:
Files in This Item:
There are no files associated with this item.

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share