Browse

Migration and Proliferation of Intact and Genetically Modified Primordial Germ Cells and the Generation of a Transgenic Chicken

Cited 27 time in Web of Science Cited 28 time in Scopus
Authors
Kim, Jin Nam; Park, Tae Sub; Park, Sang Hyun; Park, Kyung Je; Kim, Tae Min; Lee, Seul Ki; Lim, Jeong Mook; Han, Jae Yong
Issue Date
2010
Publisher
Society for the Study of Reproduction
Citation
Biology of Reproduction, vol.82 no.2, pp. 257-262
Keywords
chickenembryofemale reproductive tractgamete biologygermline chimeramigrationprimordial germ cellstransgenesis
Abstract
This study evaluated gonadal migration and postmigratory proliferation of intact and genetically modified chicken primordial germ cells (PGCs). A randomized, controlled trial was conducted with the gonadal population of PGCs and transgenic chicken production as major parameters. PGCs (0, 90, 900, 1800, or 3000 cells) were transferred into 53-h-old embryos. The percentage of PGCs migrating on Day 6 of development was highest (35.8%) following the transfer of 900 PGCs and did not change with increases in transferred PGCs. The number of migrating PGCs gradually increased (P ¼ 0.0001) as the number of transferred PGCs was increased. Gonadal migration was detected after the transfer of intact and genetically modified PGCs, but prominent decreases in PGC migration (from 21.9% to 0.38%) and chimera ratio (from 0.4 to 0.007) occurred with genetically modified PGCs. However, subsequent vigorous proliferation of the modified PGCs (3.67-fold increase from transferred number) led to the derivation of a germline chimera and produced a transgenic hatchling. In conclusion, the number of migrating PGCs increased as the number of transferred cells increased. Vigorous proliferation after transfer compensated for the decreased migration capacity of genetically modified PGCs and resulted in the production of a transgenic chicken.
ISSN
0006-3363
Language
English
URI
https://hdl.handle.net/10371/100282
DOI
https://doi.org/10.1095/biolreprod.109.079723
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse