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Production of Biofunctional Recombinant Human Interleukin 1 Receptor Antagonist (rhIL1RN) from Transgenic Quail Egg White
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kwon, Se Chang | - |
dc.contributor.author | Choi, Jin Won | - |
dc.contributor.author | Jang, Hyun-Jun | - |
dc.contributor.author | Shin, Sang Su | - |
dc.contributor.author | Lee, Seul Ki | - |
dc.contributor.author | Park, Tae Sub | - |
dc.contributor.author | Choi, In Young | - |
dc.contributor.author | Lee, Gwan Sun | - |
dc.contributor.author | Song, Gwonhwa | - |
dc.contributor.author | Han, Jae Yong | - |
dc.date.accessioned | 2017-01-31T04:36:04Z | - |
dc.date.available | 2017-01-31T04:36:04Z | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | Biology of Reproduction, vol.82 no.6, pp. 1057-1064 | ko_KR |
dc.identifier.issn | 0006-3363 | - |
dc.identifier.uri | https://hdl.handle.net/10371/100287 | - |
dc.description.abstract | Oviduct-specific expression of heterologous recombinant proteins in transgenic birds is a promising technology for the large-scale production of therapeutic proteins in eggs. We describe the production of recombinant human interleukin 1 receptor antagonist (rhIL1RN) in the eggs of transgenic quails. To drive tissue-specific expression of rhIL1RN, a 1.35-kb fragment of the chicken ovalbumin promoter, which contains both the steroid-dependent regulatory element and the negative regulatory element, was used. A transgenic quail was generated by microinjection of a concentrated stock of lentivirus into stage X blastodermal cells. A single copy of the transgene was integrated into the seventh intron of the gene for conserved oligomeric golgi complex protein 5 (COG5) on chromosome 1. As expected, rhIL1RN expression was restricted to oviductal tissue, and the amount of protein deposited in the eggs of homozygous transgenic quails ranged from 88.7 to 233.8 ng/ml. Transgene expression was conserved from the G1 generation to the G4 generation, and there was no evidence of transgene silencing. In a bioassay using the EL4.NOB-1/CTLL-2 coculture system, no significant difference was observed between the egg-produced rhIL1RN and a commercially available rhIL1RN (anakinra). | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | Society for the Study of Reproduction | ko_KR |
dc.subject | chicken ovalbumin promoter | ko_KR |
dc.subject | embryo | ko_KR |
dc.subject | gene regulation | ko_KR |
dc.subject | lentiviral vector | ko_KR |
dc.subject | rhIL1RN | ko_KR |
dc.subject | oviduct | ko_KR |
dc.subject | transgenic quail | ko_KR |
dc.title | Production of Biofunctional Recombinant Human Interleukin 1 Receptor Antagonist (rhIL1RN) from Transgenic Quail Egg White | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 권세창 | - |
dc.contributor.AlternativeAuthor | 최진원 | - |
dc.contributor.AlternativeAuthor | 장현준 | - |
dc.contributor.AlternativeAuthor | 신상수 | - |
dc.contributor.AlternativeAuthor | 이슬기 | - |
dc.contributor.AlternativeAuthor | 박태섭 | - |
dc.contributor.AlternativeAuthor | 최인영 | - |
dc.contributor.AlternativeAuthor | 이관순 | - |
dc.contributor.AlternativeAuthor | 송권화 | - |
dc.contributor.AlternativeAuthor | 한재용 | - |
dc.identifier.doi | 10.1095/biolreprod.109.081687 | - |
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