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Increased expression of cysteine cathepsins in ovarian tissue from chickens with ovarian cancer

Cited 17 time in Web of Science Cited 17 time in Scopus
Authors
Ahn, Suzie E; Choi, Jin Won; Rengaraj, Deivendran; Seo, Hee Won; Lim, Whasun; Han, Jae Yong; Song, Gwonhwa
Issue Date
2010
Publisher
BioMed Central
Citation
Reproductive Biology and Endocrinology, 8:100
Description
This is an Open Access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited.
Abstract
Background: Cysteine cathepsins (CTSs) are involved in the degradation and remodeling of the extracellular matrix
and are associated with cell transformation, differentiation, motility, and adhesion. These functions are also related
to cancer cell invasion and metastasis. Chickens spontaneously develop epithelial ovarian cancer and are therefore
a good animal model for human ovarian cancer. However, no studies have investigated the expression of CTSs in
chickens with ovarian cancer.
Methods: Cancerous (n = 5) and normal (n = 3) ovaries were collected from 2-to 3-year-old hens, and ovarian
tissue samples were collected for study. Ovarian cancers were evaluated with hematoxylin and eosin staining.
Reverse transcriptase and quantitative PCR analyses, in situ hybridization analysis were performed to examine the
mRNA expression pattern of three CTSs in detail, and protein expression of CTSB was evaluated.
Results: The CTSB, CTSC, and CTSS genes were highly expressed in cancerous chicken ovaries. Messenger RNAs for
the three CTSs were localized to a nodule area, a major characteristic of cancerous ovaries, but the three CTSs
showed no specific localization in normal ovaries. Immunoreactive CTSB protein was present in the nodule area of
cancerous ovaries.
Conclusion: Our results suggest that CTSB, CTSC, and CTSS have important functions in the development of epithelial ovarian cancer
ISSN
1477-7827
Language
English
URI
https://hdl.handle.net/10371/100288
DOI
https://doi.org/10.1186/1477-7827-8-100
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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