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Discovery of Candidate Genes and Pathways Regulating Oviduct Development in Chickens

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dc.contributor.authorSong, Gwonhwa-
dc.contributor.authorSeo, Hee Won-
dc.contributor.authorChoi, Jin Won-
dc.contributor.authorRengaraj, Deivendran-
dc.contributor.authorKim, Tae Min-
dc.contributor.authorLee, Bo Ram-
dc.contributor.authorKim, Young Min-
dc.contributor.authorYun, Tae Won-
dc.contributor.authorJeong, Jae-Wook-
dc.contributor.authorHan, Jae Yong-
dc.date.accessioned2017-01-31T06:18:15Z-
dc.date.available2017-01-31T06:18:15Z-
dc.date.issued2011-
dc.identifier.citationBiology of Reproduction, vol.85 no.2, pp. 306-314ko_KR
dc.identifier.issn0006-3363-
dc.identifier.urihttps://hdl.handle.net/10371/100308-
dc.description.abstractEstrogen is a key regulator in the development of the female reproductive system. It also stimulates oviduct development in immature chicks. We identified candidate genes and pathways associated with the development of chicken oviducts. A pellet containing the synthetic estrogen analog diethylstilbestrol (DES) was implanted subcutaneously in 1-wk-old female chicks for 10 days. The pellet was removed from half the group for 10 days, and an additional dose was given for a further 10 days. Total RNA was extracted from the oviducts of DES-treated and untreated chicks and subjected to an Affymetrix chicken GeneChip analysis. We found differential expression of 2290 and 1745 transcripts from the oviducts that were treated with DES once and twice, respectively. We also found a twofold or greater change in the expression of 77 and 390 transcripts between the two control and DES-treated time points, respectively, while we found a change in the expression of 10 transcripts that were common to all groups. Analyses of real-time PCR and in situ hybridization of selected genes confirmed the validity of the gene expression patterns observed in the microarray analysis. In particular, CCRN4L, FAM26F, HAS2, NELF, and NTM were upregulated in the DES-treated chicken oviducts. High-throughput analysis revealed that the differentially expressed genes were related to tubular formation, epithelial differentiation, hormone interactions, nerve development, and tissue remodeling in the chicken oviduct. This study provides novel insights into candidate genes regulating oviduct development and differentiation via estrogen. The identified genes may serve as biomarkers of reproductive tract development in chicks.ko_KR
dc.language.isoenko_KR
dc.publisherSociety for the Study of Reproductionko_KR
dc.subjectchickenko_KR
dc.subjectdiethylstilbestrolko_KR
dc.subjectestradiol/estradiol receptorko_KR
dc.subjectestrogenko_KR
dc.subjectfemale reproductive tractko_KR
dc.subjectmicroarray analysisko_KR
dc.subjectoviductko_KR
dc.subjectuterusko_KR
dc.titleDiscovery of Candidate Genes and Pathways Regulating Oviduct Development in Chickensko_KR
dc.typeArticleko_KR
dc.identifier.doi10.1095/biolreprod.110.089227-
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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