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Characterization and Application of Oviductal Epithelial Cells In Vitro in Gallus domesticus

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dc.contributor.authorJung, Jin Gyoung-
dc.contributor.authorPark, Tae Sub-
dc.contributor.authorKim, Jin Nam-
dc.contributor.authorHan, Beom Ku-
dc.contributor.authorLee, Seon Duk-
dc.contributor.authorSong, Gwonhwa-
dc.contributor.authorHan, Jae Yong-
dc.date.accessioned2017-01-31T06:19:00Z-
dc.date.available2017-01-31T06:19:00Z-
dc.date.issued2011-
dc.identifier.citationBiology of Reproduction, vol.85 no.4, pp. 798-807ko_KR
dc.identifier.issn0006-3363-
dc.identifier.urihttps://hdl.handle.net/10371/100311-
dc.description.abstractChicken oviductal epithelium produces large quantities of egg white protein in daily cycles. In this study, we cultured and characterized oviductal epithelial cells (OECs) from juvenile (10-wk-old) chickens and from actively laying (30-wk-old) hens. The juvenile OECs were maintained over passage 25 and were positive for toluidine blue, lectin-ConA, HPA, UEA-1, WFA, WGA, anti-OVA, anti-ESR1, and anti-PGR, whereas the adult OECs were cultured over passage 6 and were positive for toluidine blue, periodic acid-Schiff, lectin-ConA, WFA, WGA, anti-OVA, anti-ESR1, and anti-PGR. To investigate the optimal concentration of steroid hormones for inducing egg white protein genes in vitro, we examined the effects of estrogen, diethylstilbestrol, progesterone, and corticosterone on OECs. Results showed that oviduct-specific levels of avidin, ovalbumin, ovomucin, lysozyme, ESR1, and PGR gene expression were significantly elevated in steroid hormone-treated OECs compared with those of untreated cells (P , 0.05). Ovalbumin protein was also secreted into culture medium from hormonetreated OECs. In addition, to examine the application of OECs for avian transgenesis, we introduced human thrombopoietin (THPO)-expressing lentiviral vector controlled by a 3.5-kb ovalbumin promoter into cultured OECs, and THPO expression was significantly induced with diethylstilbestrol or progesterone in juvenile OECs (P , 0.05) and in adult OECs (P , 0.05). In conclusion, these data demonstrate the potential of cultured OECs as a model system for providing a better understanding of the regulation of gene expression and for the production of an avian transgenic bioreactor.ko_KR
dc.language.isoenko_KR
dc.publisherSociety for the Study of Reproductionko_KR
dc.subjectchickenko_KR
dc.subjectepithelial cellsko_KR
dc.subjectlectinko_KR
dc.subjectoviductko_KR
dc.subjecttubular gland cellsko_KR
dc.titleCharacterization and Application of Oviductal Epithelial Cells In Vitro in Gallus domesticusko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor정진경-
dc.contributor.AlternativeAuthor박태섭-
dc.contributor.AlternativeAuthor김진남-
dc.contributor.AlternativeAuthor한범구-
dc.contributor.AlternativeAuthor이선덕-
dc.contributor.AlternativeAuthor송권화-
dc.contributor.AlternativeAuthor한재용-
dc.identifier.doi10.1095/biolreprod.111.092023-
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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