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Cell-Specific and Temporal Aspects of Gene Expression in the Chicken Oviduct at Different Stages of the Laying Cycle

Cited 14 time in Web of Science Cited 14 time in Scopus
Authors
Jeong, Wooyoung; Lim, Whasun; Kim, Jinyoung; Ahn, Suzie E.; Lee, Hyung Chul; Jeong, Jae-Wook; Han, Jae Yong; Song, Gwonhwa; Bazer, Fuller W.
Issue Date
2012
Publisher
Society for the Study of Reproduction
Citation
Biology of Reproduction, vol.86 no.6, pp. 172-179
Keywords
chickeneggmicroarray analysisoviductovulation
Abstract
Egg formation and embryonic development occur as the yolk passes through the magnum, isthmus, and shell gland of the oviduct before oviposition in hens. The present study identified candidate genes associated with secretory function of the chicken oviduct after ovulation and contributing to egg formation and oviposition. Hens (n ¼ 5 per time point) were euthanized to recover the reproductive tract when the egg was in the magnum (3 h after ovulation) and the shell gland (20 h after ovulation). Total RNA was extracted from each segment of the oviducts and subjected to Affymetrix chicken GeneChip analysis. Quantitative PCR and in situ hybridization analyses of selected genes confirmed the validity of the gene expression patterns detected using microarray analysis. In particular, ACP1, CALB1, CYP26A1, PENK, RCAN1 and SPP1 expression increased significantly in the shell gland between 3 h and 20 h postovulation, whereas only RCNA1 expression increased significantly in the magnum between 3 h and 20 h postovulation. Results of the high-throughput analysis revealed cell-specific and temporal changes in gene expression in the oviduct at 3 h and 20 h postovulation in laying hens provide novel insight into changes at the molecular and cellular levels of candidate genes related to formation of the egg and oviposition.
ISSN
0006-3363
Language
English
URI
https://hdl.handle.net/10371/100333
DOI
https://doi.org/10.1095/biolreprod.111.098186
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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