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Effects of a single nucleotide polymorphism in the chicken NK-lysin gene on antimicrobial activity and cytotoxicity of cancer cells

Cited 32 time in Web of Science Cited 34 time in Scopus
Authors
Lee, Mi Ok; Kim, Eun-Hee; Jang, Hyun-Jun; Park, Mi Na; Woo, Hee-Jong; Han, Jae Yong; Womack, James E.
Issue Date
2012
Publisher
National Academy of Sciences
Citation
Proceedings of the National Academy of Sciences, vol.109 no.30, pp. 12087-12092
Keywords
antimicrobial peptidesgenetic variationinnate immunity
Abstract
NK-lysin is an effector protein of the innate immune system and an important component of host protection. We isolated a SNP in the NK-lysin coding sequence among different chicken breeds. This A to G substitution at the position 271 nucleotide in the ORF results in an Asn (N) to Asp (D) amino acid alteration. We synthesized two 30-aa peptides (N29N and N29D) to compare the biological activity of the helix 2-loop-helix 3 region of NK-lysin resulting from the polymorphic gene. Both peptides were found to be cytotoxic in bacteria and tumor cell cultures at micromolar concentrations. The N29N peptide, however, exhibited greater antibacterial and anticancer activity than the N29D peptide. Circular dichroism spectroscopy of the two peptides in negatively charged single unilamellar vesicles showed spectra typical of α-helical peptides. The helical profile of N29D was reduced substantially compared with that of N29N. However, no structural change was observed in neutral vesicles. ζ-Potential measurements of liposomes incubated with increasing peptide concentrations allowed surface charge neutralization with a negatively charged lipid, but not with a zwitterionic lipid. This result suggests that a difference in electrostatic interaction between lipid membranes and the helical peptides results from the polymorphic gene and is subsequently an important factor in cell lytic activity of variant NK-lysin peptides.
ISSN
0027-8424
Language
English
URI
https://hdl.handle.net/10371/100337
DOI
https://doi.org/10.1073/pnas.1209161109
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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