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Compensatory proliferation of endogenous chicken primordial germ cells after elimination by busulfan treatment
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Hyung Chul | - |
dc.contributor.author | Kim, Sung Kyu | - |
dc.contributor.author | Park, Tae Sub | - |
dc.contributor.author | Rengaraj, Deivendran | - |
dc.contributor.author | Park, Kyung Je | - |
dc.contributor.author | Lee, Hong Jo | - |
dc.contributor.author | Park, Soo Bong | - |
dc.contributor.author | Kim, Sung Woo | - |
dc.contributor.author | Choi, Seong Bok | - |
dc.contributor.author | Han, Jae Yong | - |
dc.date.accessioned | 2017-02-02T01:45:06Z | - |
dc.date.available | 2017-02-02T01:45:06Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Stem Cell Research and Therapy, 4:136 | ko_KR |
dc.identifier.issn | 1757-6512 | - |
dc.identifier.uri | http://stemcellres.com/content/4/6/136 | - |
dc.identifier.uri | https://hdl.handle.net/10371/100371 | - |
dc.description | This is an open access article distributed under the terms of the Creative
Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | ko_KR |
dc.description.abstract | Introduction
Primordial germ cells (PGCs) are the major population of cells in the developing bilateral embryonic gonads. Little is known about the cellular responses of PGCs after treatment with toxic chemicals such as busulfan during embryo development. In this study, we investigated the elimination, restorative ability, and cell cycle status of endogenous chicken PGCs after busulfan treatment. Methods Busulfan was emulsified in sesame oil by a dispersion-emulsifying system and injected into the chick blastoderm (embryonic stage X). Subsequently, we conducted flow cytometry analysis to evaluate changes in the PGC population and cell cycle status, and immunohistochemistry to examine the germ cell proliferation. Results Results of flow cytometry and immunohistochemistry analyses after busulfan treatment showed that the proportion of male PGCs at embryonic day 9 and female PGCs at embryonic day 7 were increased by approximately 60% when compared with embryonic day 5.5. This result suggests the existence of a compensatory mechanism in PGCs in response to the cytotoxic effects of busulfan. Results of cell cycling analysis showed that the germ cells in the G0/G1 phase were significantly decreased, while S/G2/M-phase germ cells were significantly increased in the treatment group compared with the untreated control group in both 9-day-old male and female embryos. In addition, in the proliferation analysis with 5-ethynyl-2′-deoxyuridine (EdU) incorporation, we found that the proportion of EdU-positive cells among VASA homolog-positive cells in the 9-day embryonic gonads of the busulfan-treated group was significantly higher than in the control group. Conclusions We conclude that PGCs enter a restoration pathway by promoting their cell cycle after experiencing a cytotoxic effect. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BioMed Central | ko_KR |
dc.title | Compensatory proliferation of endogenous chicken primordial germ cells after elimination by busulfan treatment | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 이형철 | - |
dc.contributor.AlternativeAuthor | 김성규 | - |
dc.contributor.AlternativeAuthor | 박태섭 | - |
dc.contributor.AlternativeAuthor | 박경제 | - |
dc.contributor.AlternativeAuthor | 이홍조 | - |
dc.contributor.AlternativeAuthor | 박수봉 | - |
dc.contributor.AlternativeAuthor | 김성우 | - |
dc.contributor.AlternativeAuthor | 최성복 | - |
dc.contributor.AlternativeAuthor | 한재용 | - |
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