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Production of egg yolk antibodies specific to house dust mite proteins

DC Field Value Language
dc.contributor.authorLee, Kyung Eun-
dc.contributor.authorHan, Beom Ku-
dc.contributor.authorHan, Jae Yong-
dc.contributor.authorHong, Jung Yeon-
dc.contributor.authorKim, Mi Na-
dc.contributor.authorHeo, Won Il-
dc.contributor.authorSohn, Myung Hyun-
dc.contributor.authorKim, Kyung Won-
dc.contributor.authorKim, Kyu Earn-
dc.date.accessioned2017-02-02T02:14:00Z-
dc.date.available2017-02-02T02:14:00Z-
dc.date.issued2014-
dc.identifier.citationYonsei Medical Journal, vol.55 no.4, pp. 999-1004ko_KR
dc.identifier.issn0513-5796-
dc.identifier.urihttps://hdl.handle.net/10371/100378-
dc.descriptionThis is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/ licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.ko_KR
dc.description.abstractPurpose
House dust mites (HDMs) are an important source of indoor allergens associated with asthma, rhinitis and atopic dermatitis. Chicken immunoglobulin (Ig) Y is known to be a good alternative to mice and rabbit antibody production. In this study, we produced IgYs specific to HDMs and investigated their IgE immunoreactivities.

Materials and Methods
Total IgYs were isolated from the yolks of White Leghorn hens immunized with either Dermatophagoides pteronyssinus or D. farinae protein extract. Control antibodies were separated from the yolks of immunized hens with phosphate buffered saline. IgYs specific to HDMs were analyzed using enzyme-linked immunosorbent assay and Western blotting analysis.

Results
The concentration of egg IgY specific to D. farinae in an immunized hen increased and the highest achieved was 661.3 ug/mg (per an egg) on day 47, compared with 760 ug/mg IgY specific to D. pteronyssinus on day 16. The D. pteronyssinus or D. farinae-specific IgY was detected by binding of each mite proteins, and their immunoreactivities were elevated dependent of the specific IgY concentration.

Conclusion
IgY specific to HDMs may be a promising antibody for immunological diagnosis as well as identification of possible resistance relating to HDM allergy.
ko_KR
dc.language.isoenko_KR
dc.publisherYonsei University College of Medicineko_KR
dc.subjectHouse dust miteko_KR
dc.subjectimmunoglobulin Yko_KR
dc.subjectD. pteronyssinusko_KR
dc.subjectD. farinaeko_KR
dc.titleProduction of egg yolk antibodies specific to house dust mite proteinsko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor이경은-
dc.contributor.AlternativeAuthor한범구-
dc.contributor.AlternativeAuthor한재용-
dc.contributor.AlternativeAuthor홍정연-
dc.contributor.AlternativeAuthor김미나-
dc.contributor.AlternativeAuthor허원일-
dc.contributor.AlternativeAuthor손명현-
dc.contributor.AlternativeAuthor김경원-
dc.contributor.AlternativeAuthor김규언-
dc.identifier.doi10.3349/ymj.2014.55.4.999-
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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