Publications
Detailed Information
Production of Interspecific Germline Chimeras via Embryo Replacement
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Hee Jung | - |
dc.contributor.author | Lee, Hyung Chul | - |
dc.contributor.author | Kang, Kyung Soo | - |
dc.contributor.author | Lee, Hyo Gun | - |
dc.contributor.author | Ono, Tamao | - |
dc.contributor.author | Nagai, Hiroki | - |
dc.contributor.author | Sheng, Guojun | - |
dc.contributor.author | Han, Jae Yong | - |
dc.date.accessioned | 2017-02-06T01:50:01Z | - |
dc.date.available | 2017-02-06T01:50:01Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Biology of Reproduction, vol.93 no.2, pp. 36-42 | ko_KR |
dc.identifier.issn | 0006-3363 | - |
dc.identifier.uri | https://hdl.handle.net/10371/100425 | - |
dc.description | This is an Open Access article, freely available through Biology of Reproductions Authors Choice option. | ko_KR |
dc.description.abstract | In avian species, primordial germ cells (PGCs) use the vascular system to reach their destination, the genital ridge. Because of this unique migratory route of avian germ cells, germline chimera production can be achieved via germ cell transfer into a blood vessel. This study was performed to establish an alternative germ cell-transfer system for producing germline chimeras by replacing an original host embryo with a donor embryo, while retaining the host extraembryonic tissue and yolk, before circulation. First, to test the migratory capacity of PGCs after embryo replacement, Korean Oge (KO) chick embryos were used to replace GFP transgenic chick embryos. Four days after replacement, GFP-positive cells were detected in the replaced KO embryonic gonads, and genomic DNA PCR analysis with the embryonic gonads demonstrated the presence of the GFP transgene. To produce an interspecific germline chimera, the original chick embryo proper was replaced with a quail embryo onto the chick yolk. To detect the gonadal PGCs in the 5.5-day-old embryonic gonads, immunohistochemistry was performed with monoclonal antibodies specific to either quail or chick PGCs, i.e., QCR1 and anti-stage-specific embryonic antigen-1 (SSEA-1), respectively. Both the QCR1-positive and SSEA-1-positive cells were detected in the gonads of replaced quail embryos. Forty percent of the PGC population in the quail embryos was occupied by chick extraembryonically derived PGCs. In conclusion, replacement of an embryo onto the host yolk before circulation can be applied to produce interspecies germline chimeras, and this germ cell-transfer technology is potentially applicable for reproduction of wild or endangered bird species. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | Society for the Study of Reproduction | ko_KR |
dc.subject | chicken | ko_KR |
dc.subject | embryo replacement | ko_KR |
dc.subject | interspecies germline chimera | ko_KR |
dc.subject | primordial germ cell | ko_KR |
dc.subject | quail | ko_KR |
dc.title | Production of Interspecific Germline Chimeras via Embryo Replacement | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 최희준 | - |
dc.contributor.AlternativeAuthor | 이형철 | - |
dc.contributor.AlternativeAuthor | 강경수 | - |
dc.contributor.AlternativeAuthor | 이효근 | - |
dc.contributor.AlternativeAuthor | 한재용 | - |
dc.identifier.doi | 10.1095/biolreprod.114.127365 | - |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.