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SP, CGRP changes in pyridoxine induced neuropathic dogs with nerve growth factor gene therapy

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Authors
Kang, Joo-Yeon; Yoo, Dae Young; Lee, Kwon-Young; Im, Wooseok; Kim, Manho; Choi, Jung Hoon; Youn, Hwa-Young; Kim, Sae Hoon; Hwang, In Koo; Chung, Jin-Young
Issue Date
2016-01-05
Publisher
BioMed Central
Citation
BMC Neuroscience, 17(1):1
Keywords
Calcitonin gene-related peptideDogH reflexNerve growth factorPyridoxineNeuropathy
Description
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License
(http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium,
provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license,
and indicate if changes were made.
Abstract
Abstract

Background
Nerve growth factor (NGF) is known not only as a major factor for neuronal plasticity but also as a pain stimulator. Although there have been several trials with NGF for its application in the regeneration or protection of the nervous system, the pain induced by NGF remains a challenge to be overcome. In this study, the pain induced by NGF gene therapy was evaluated.


Results
Vehicle or recombinant dog NGF plasmid was administered into the intrathecal space of dogs. Twenty-four hours after the vehicle or NGF plasmid inoculation, dogs were subcutaneously treated with 150 mg/kg pyridoxine every day for 7 days. For pain assessment, physical examination and electrophysiological recording were performed. Only in the vehicle-treated group, weight loss occurred, while NGF plasmid inoculation significantly improved this physical abnormalities. In the vehicle-treated group, electrophysiological recordings showed that H-reflex disappeared at 24 h after the last pyridoxine treatment. However, in the NGF plasmid inoculated group, the H-reflex were normal. In the results of immunohistochemistry, the NGF plasmid administration efficiently expressed in the dorsal root ganglia and significantly increased the pyridoxine-induced reduction of calcitonin gene-related peptide (CGRP) immunoreactive neurons, but not in substance P immunoreactive neurons, in the dorsal root ganglia.


Conclusions
Given these results, we reason that NGF gene therapy in pyridoxine induced neuropathic dogs does not induce neuropathic pain with this dosage, even with increasing the expression of CGRP.
Language
English
URI
http://hdl.handle.net/10371/100654
DOI
https://doi.org/10.1186/s12868-015-0236-5
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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