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Labeling efficacy of superparamagnetic iron oxide nanoparticles to human neural stem cells: comparison of ferumoxides, monocrystalline iron oxide, cross-linked iron oxide (CLIO)-NH2 and tat-CLIO

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Authors
Song, Miyeoun; Moon, Woo Kyung; Kim, Yunhee; Lim, Dongyeol; Song, In-Chan; Yoon, Byung-Woo
Issue Date
2007-10-10
Publisher
The Korean Radiological Society
Citation
Korean J Radiol 2007;8(5):365-371
Keywords
Cells, CulturedContrast Media/chemical synthesis/pharmacokineticsCross-Linking Reagents/chemistryFerric Compounds/chemistry/*pharmacokineticsFerrosoferric Oxide/chemical synthesis/pharmacokineticsGene Products, tat/chemistryHumansIron/*pharmacokineticsMagnetic Resonance Imaging/methodsNanoparticlesNeural TubeOxides/*pharmacokineticsPhantoms, ImagingPolylysine/pharmacokineticsSpectrophotometry, AtomicStaining and Labeling/*methodsStem Cells/cytology/*drug effects/metabolismTime FactorsTransfection
Abstract
OBJECTIVE: We wanted to compare the human neural stem cell (hNSC) labeling efficacy of different superparamagnetic iron oxide nanoparticles (SPIONs), namely, ferumoxides, monocrystalline iron oxide (MION), cross-linked iron oxide (CLIO)-NH(2) and tat-CLIO. MATERIALS AND METHODS: The hNSCs (5 x 10(5) HB1F3 cells/ml) were incubated for 24 hr in cell culture media that contained 25 microg/ml of ferumoxides, MION or CLIO-NH(2), and with or without poly-L-lysine (PLL) and tat-CLIO. The cellular iron uptake was analyzed qualitatively with using a light microscope and this was quantified via atomic absorption spectrophotometry. The visibility of the labeled cells was assessed with MR imaging. RESULTS: The incorporation of SPIONs into the hNSCs did not affect the cellular proliferations and viabilities. The hNSCs labeled with tat-CLIO showed the longest retention, up to 72 hr, and they contained 2.15+/-0.3 pg iron/cell, which are 59 fold, 430 fold and six fold more incorporated iron than that of the hNSCs labeled with ferumoxides, MION or CLIO-NH(2), respectively. However, when PLL was added, the incorporation of ferumoxides, MION or CLIO-NH(2) into the hNSCs was comparable to that of tat-CLIO. CONCLUSION: For MR imaging, hNSCs can be efficiently labeled with tat-CLIO alone or with a combination of ferumoxides, MION, CLIO-NH(2) and the transfection agent PLL.
ISSN
1229-6929 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17923778

https://hdl.handle.net/10371/10336
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Radiation Applied Life Science (대학원 협동과정 방사선응용생명과학전공)Journal Papers (저널논문_방사선응용생명과학전공)
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