S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Radiation Applied Life Science (대학원 협동과정 방사선응용생명과학전공) Journal Papers (저널논문_방사선응용생명과학전공)
Labeling efficacy of superparamagnetic iron oxide nanoparticles to human neural stem cells: comparison of ferumoxides, monocrystalline iron oxide, cross-linked iron oxide (CLIO)-NH2 and tat-CLIO
- Song, Miyeoun; Moon, Woo Kyung; Kim, Yunhee; Lim, Dongyeol; Song, In-Chan; Yoon, Byung-Woo
- Issue Date
- The Korean Radiological Society
- Korean J Radiol 2007;8(5):365-371
- Cells, Cultured; Contrast Media/chemical synthesis/pharmacokinetics; Cross-Linking Reagents/chemistry; Ferric Compounds/chemistry/*pharmacokinetics; Ferrosoferric Oxide/chemical synthesis/pharmacokinetics; Gene Products, tat/chemistry; Humans; Iron/*pharmacokinetics; Magnetic Resonance Imaging/methods; Nanoparticles; Neural Tube; Oxides/*pharmacokinetics; Phantoms, Imaging; Polylysine/pharmacokinetics; Spectrophotometry, Atomic; Staining and Labeling/*methods; Stem Cells/cytology/*drug effects/metabolism; Time Factors; Transfection
- OBJECTIVE: We wanted to compare the human neural stem cell (hNSC) labeling efficacy of different superparamagnetic iron oxide nanoparticles (SPIONs), namely, ferumoxides, monocrystalline iron oxide (MION), cross-linked iron oxide (CLIO)-NH(2) and tat-CLIO. MATERIALS AND METHODS: The hNSCs (5 x 10(5) HB1F3 cells/ml) were incubated for 24 hr in cell culture media that contained 25 microg/ml of ferumoxides, MION or CLIO-NH(2), and with or without poly-L-lysine (PLL) and tat-CLIO. The cellular iron uptake was analyzed qualitatively with using a light microscope and this was quantified via atomic absorption spectrophotometry. The visibility of the labeled cells was assessed with MR imaging. RESULTS: The incorporation of SPIONs into the hNSCs did not affect the cellular proliferations and viabilities. The hNSCs labeled with tat-CLIO showed the longest retention, up to 72 hr, and they contained 2.15+/-0.3 pg iron/cell, which are 59 fold, 430 fold and six fold more incorporated iron than that of the hNSCs labeled with ferumoxides, MION or CLIO-NH(2), respectively. However, when PLL was added, the incorporation of ferumoxides, MION or CLIO-NH(2) into the hNSCs was comparable to that of tat-CLIO. CONCLUSION: For MR imaging, hNSCs can be efficiently labeled with tat-CLIO alone or with a combination of ferumoxides, MION, CLIO-NH(2) and the transfection agent PLL.
- 1229-6929 (Print)
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