S-Space College of Medicine/School of Medicine (의과대학/대학원) Urology (비뇨기과학전공) Journal Papers (저널논문_비뇨기과학전공)
Monitoring transplanted human mesenchymal stem cells in rat and rabbit bladders using molecular magnetic resonance imaging
- Issue Date
- Neurourol Urodyn. 2007;26(4):584-93.
- Adipocytes/physiology ; Animals ; Cell Differentiation/physiology ; Cell Survival/physiology ; Chondrocytes/physiology ; Ferric Compounds/diagnostic use ; Humans ; Magnetic Resonance Imaging ; Nanoparticles/diagnostic use ; Osteocytes/physiology ; Rabbits ; Rats ; Urinary Bladder/*cytology/*physiology ; Mesenchymal Stem Cell Transplantation
- AIMS: This study investigated whether superparamagnetic iron oxide (SPIO)-labeled human mesenchymal stem cells (hMSCs) may be monitored non-invasively by in vivo magnetic resonance (MR) imaging with conventional 1.5-T system examinations in the bladders of rats and rabbits. METHODS: SPIO were transferred to hMSCs, using GenePORTER. After SPIO-labeled hMSCs were transplanted into the animal bladders, serial T2-weighted MR images and histological examinations were performed over a 4-week period. RESULTS: hMSCs loaded with SPIO, compared to unlabeled cells, showed similar viability. SPIO-labeled hMSCs underwent normal chondrogenic, adipogenic, and osteogenic differentiation. For SPIO-labeled hMSCs concentrations that were greater than 1x10(5), in vitro MR images showed a decrease in signal intensity. MR signal intensity at the areas of SPIO-labeled hMSCs in rat and rabbit bladders were decreased and confined locally. After injection of SPIO-labeled hMSCs into the bladder, MR imaging demonstrated that hMSCs could be seen for at least 12 weeks post-injection. The presence of iron was confirmed with Prussian blue staining in histological sections. CONCLUSIONS: Our findings suggest that hMSCs in animal bladders can be monitored non-invasively with conventional MR imaging.
- 0733-2467 (Print)
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