Browse

Plasma glutathione reductase activity and prognosis of septic shock
26316444

Cited 12 time in Web of Science Cited 11 time in Scopus
Issue Date
2015-08-05
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
JOURNAL OF SURGICAL RESEARCH Vol.200 No.1, pp. 298-307
Keywords
Septic shockOxidative stressGlutathioneOxidation reductionGlutathione reductaseMortality
Abstract
Background: Our aim was to investigate whether plasma glutathione reductase (GR) activity is well correlated with the erythrocyte-reduced glutathione (GSH)/glutathione disulfide (GSSG) ratio and is associated with the mortality of septic shock.
Materials and methods: This study was conducted on male Sprague-Dawley rats and patients admitted to the intensive care unit with septic shock. To induce endotoxemia in rats, vehicle or lipopolysaccharide (LPS) at dosages of 5 or 10 mg/kg were injected into a tail vein. Animals were then euthanized 6 h post-LPS. Based on the 28-d mortality, the enrolled patients were divided into the survivors and nonsurvivors. We obtained blood samples from patients at admission (0 h) and 24 h after admission to the intensive care unit.
Results: In endotoxemic rats, the erythrocyte GSH/GSSG ratio, erythrocyte GR activity, and plasma GR activity in the 10 mg/kg of LPS group were lower than those in the sham and 5 mg/kg of LPS groups. In patients with septic shock, decrease in plasma GR activity at 24 h was independently associated with an increase in 28-d mortality (odds ratio, 0.828; 95% confidence interval, 0.690-0.992, P ¼ 0.041). Plasma GR activity was correlated with erythrocyte GR activity (Spearman r ¼ 0.549, P < 0.001) and the erythrocyte GSH/GSSG ratio (rho ¼ 0.367, P ¼ 0.009) at 24 h.
Conclusions: Plasma GR activity was well correlated with erythrocyte GR activity and the erythrocyte GSH/GSSG ratio, and a decrease in plasma GR activity was associated with an increase in the mortality of septic shock patients.
ISSN
0022-4804
Language
English
URI
https://hdl.handle.net/10371/116924
DOI
https://doi.org/10.1016/j.jss.2015.07.044
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)Journal Papers (저널논문_의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse