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Soluble expression and stability enhancement of transcription factors using 30Kc19 cell-penetrating protein
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ryu, Jina | - |
dc.contributor.author | Park, Hee Ho | - |
dc.contributor.author | Park, Ju Hyun | - |
dc.contributor.author | Lee, Hong Jai | - |
dc.contributor.author | Rhee, Won Jong | - |
dc.contributor.author | Park, Tai Hyun | - |
dc.date.accessioned | 2017-04-21T02:30:01Z | - |
dc.date.available | 2017-07-30T23:31:53Z | - |
dc.date.created | 2018-07-09 | - |
dc.date.issued | 2016-04 | - |
dc.identifier.citation | Applied Microbiology and Biotechnology, Vol.100 No.8, pp.3523-3532 | - |
dc.identifier.issn | 0175-7598 | - |
dc.identifier.uri | https://hdl.handle.net/10371/116991 | - |
dc.description.abstract | Transcription factors have been studied as an important drug candidate. Ever since the successful generation of induced pluripotent stem cells (iPSCs), there has been tremendous interest in reprogramming transcription factors. Because of the safety risks involved in a virus-based approach, many researchers have been trying to deliver transcription factors using nonintegrating materials. Thus, delivery of transcription factors produced as recombinant proteins in E. coli was proposed as an alternative method. However, the low level of soluble expression and instability of such recombinant proteins are potential barriers. We engineered a Bombyx mori 30Kc19 protein as a fusion partner for transcription factors to overcome those problems. We have previously reported that 30Kc19 protein can be produced as a soluble form in E. coli and has a cell-penetrating property and a protein-stabilizing effect. Transcription factors fused with 30Kc19 (Oct4-30Kc19, Sox2-30Kc19, c-Myc-30Kc19, L-Myc-30Kc19, and Klf4-30Kc19) were produced as recombinant proteins. Interestingly, Oct4 and L-Myc were expressed as a soluble form by conjugating with 30Kc19 protein, whereas Oct4 alone and L-Myc alone aggregated. The 30Kc19 protein also enhanced the stability of transcription factors both in vitro and in cells. In addition, 30Kc19-conjugated transcription factors showed rapid delivery into cells and transcriptional activity significantly increased. Overall, 30Kc19 protein conjugation simultaneously enhanced soluble expression, stability, and transcriptional activity of transcription factors. We propose that the conjugation with 30Kc19 protein is a novel approach to solve the technical bottleneck of gene regulation using transcription factors. | - |
dc.language | 영어 | - |
dc.language.iso | en | en |
dc.publisher | Springer Verlag | - |
dc.title | Soluble expression and stability enhancement of transcription factors using 30Kc19 cell-penetrating protein | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 류지나 | - |
dc.contributor.AlternativeAuthor | 박희호 | - |
dc.contributor.AlternativeAuthor | 박주현 | - |
dc.contributor.AlternativeAuthor | 이홍재 | - |
dc.contributor.AlternativeAuthor | 이원종 | - |
dc.contributor.AlternativeAuthor | 박태현 | - |
dc.identifier.doi | 10.1007/s00253-015-7199-4 | - |
dc.citation.journaltitle | Applied Microbiology and Biotechnology | - |
dc.identifier.wosid | 000373744200013 | - |
dc.identifier.scopusid | 2-s2.0-84949813133 | - |
dc.description.srnd | OAIID:RECH_ACHV_DSTSH_NO:T201623709 | - |
dc.description.srnd | RECH_ACHV_FG:RR00200001 | - |
dc.description.srnd | ADJUST_YN: | - |
dc.description.srnd | EMP_ID:A002014 | - |
dc.description.srnd | CITE_RATE:3.376 | - |
dc.description.srnd | FILENAME:2. (2016.04) Soluble expression and stability enhancement of.pdf | - |
dc.description.srnd | DEPT_NM:화학생물공학부 | - |
dc.description.srnd | EMAIL:thpark@snu.ac.kr | - |
dc.description.srnd | SCOPUS_YN:Y | - |
dc.description.srnd | FILEURL:https://srnd.snu.ac.kr/eXrepEIR/fws/file/0df54ee9-e9f1-4612-9d6e-6deaa8197e3e/link | - |
dc.description.srnd | CONFIRM:Y | - |
dc.citation.endpage | 3532 | - |
dc.citation.number | 8 | - |
dc.citation.startpage | 3523 | - |
dc.citation.volume | 100 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Park, Tai Hyun | - |
dc.identifier.srnd | T201623709 | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | PLURIPOTENT STEM-CELLS | - |
dc.subject.keywordPlus | SILKWORM HEMOLYMPH | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | RECOMBINANT PROTEINS | - |
dc.subject.keywordPlus | HUMAN FIBROBLASTS | - |
dc.subject.keywordPlus | SOMATIC-CELLS | - |
dc.subject.keywordPlus | BOMBYX-MORI | - |
dc.subject.keywordPlus | APOPTOSIS | - |
dc.subject.keywordPlus | INHIBITION | - |
dc.subject.keywordPlus | SOLUBILITY | - |
dc.subject.keywordAuthor | 30Kc19 protein | - |
dc.subject.keywordAuthor | Cell-penetrating property | - |
dc.subject.keywordAuthor | Recombinant protein | - |
dc.subject.keywordAuthor | Transcription factor | - |
dc.subject.keywordAuthor | Soluble expression | - |
dc.subject.keywordAuthor | Protein stability | - |
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