S-Space College of Engineering/Engineering Practice School (공과대학/대학원) Dept. of Chemical and Biological Engineering (화학생물공학부) Journal Papers (저널논문_화학생물공학부)
Human Dopamine Receptor-Conjugated Multidimensional Conducting Polymer Nanofiber Membrane for Dopamine Detection
- Park, Seon Joo; Lee, Seung Hwan; Yang, Heehong; Park, Chul Soon; Lee, Chang-Soo; Kwon, Oh Seok; Park, Tai Hyun; Jang, Jyongsik
- Issue Date
- AMERICAN CHEMICAL SOCIETY
- ACS APPLIED MATERIALS & INTERFACES Vol.8 No.42, pp. 28897-28903
- multidimensional nanostructures; conducting polymers; human dopamine receptor; protein-based biosensor; microfluidic FET system
- In the brain and central nervous system, dopamine plays a crucial role as a neurotransmitter or a local chemical messenger for interneuronal communication. Dopamine is associated with renal, hormonal, and cardiovascular systems. Additionally, dopamine dysfunction is known to cause serious illnesses, such as Parkinson's disease and Alzheimer's disease. Therefore, dopamine detection is essential for medical diagnosis and disease prevention and requires a novel strategy with high sensitivity and selectivity and a rapid response. Herein, we present a novel human dopamine receptor (hDRD1)-conjugated multidimensional conducting polymer nanofiber (NF) membrane for the selective and sensitive detection of dopamine. The membrane, which consists of multidimensional carboxylated poly(3,4-ethylenedioxythiophene) (MCPEDOT) NFs with nanorods, is used as a transistor in a liquid-ion gated field-effect transistor (FET)-based biosensor. Interestingly, hDRD1 is first expressed in Escherichia coli before it is immobilized onto the MCPEDOT NF. The hDRD1 MCPEDOT NF -based FET exhibits a rapid real-time response (<2 s) with high dopamine selectivity and sensitivity performance (approximately 100 84). Furthermore, this FET device can be integrated into a poly(dimethylsiloxane)-based microfluidic system and also can retain its high performance in the integrated system, which results in the generation of large-scale dopamine biosensors with a novel geometry.
- Files in This Item: There are no files associated with this item.