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One-step isolation of sappanol and brazilin from Caesalpinia sappan and their effects on oxidative stress-induced retinal death

Cited 14 time in Web of Science Cited 13 time in Scopus
Authors
Uddin, Golam Mezbah; Kim, Chul Young; Chung, Donghwa; Kim, Kyung-A; Jung, Sang Hoon
Issue Date
2015-03
Publisher
Korean Society for Biochemistry and Molecular Biology
Citation
BMB Reports, Vol.48 No.5, pp. 289-294
Keywords
One-step isolation of sappanol and brazilin from Caesalpinia sappan and their effects on oxidative stress-induced retinal death자연과학AntioxidantCaesalpinia sappanCentrifugal partition chromatographyHomoisoflavonoidsN-retinylidene-N-retinyl-ethanolamine (A2E) photooxidation
Abstract
Caesalpinia sappan is a well-distributed plant that is cultivated in Southeast Asia, Africa, and the Americas. C. sappan has been used in Asian folk medicine and its extract has been shown to have pharmacological effects. Two homoisoflavonoids, sappanol and brazilin, were isolated from C. sappan by using centrifugal partition chromatography (CPC), and tested for protective effects against retinal cell death. The isolated homoisoflavonoids produced approximately 20-fold inhibition of N-retinylidene-N-retinyl-ethanolamine (A2E) photooxidation in a dose-dependent manner. Of the 2 compounds, brazilin showed better inhibition (197.93 ± 1.59 μM of IC50). Cell viability tests and PI/Hoechst 33342 double staining method indicated that compared to the negative control, sappanol significantly attenuated H2O2-induced retinal death. The compounds significantly blunted the up-regulation of intracellular reactive oxygen species (ROS), and sappanol inhibited lipid peroxidation in a concentration-dependent manner. Thus, both compounds represent potential antioxidant treatments for retinal diseases.
ISSN
1976-6696
Language
English
URI
https://hdl.handle.net/10371/117441
DOI
https://doi.org/10.5483/BMBRep.2015.48.5.189
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Graduate School of International Agricultural Technology (국제농업기술대학원)Dept. of International Agricultural Technology (국제농업기술학과)Journal Papers (저널논문_국제농업기술학과)
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