S-Space College of Medicine/School of Medicine (의과대학/대학원) Laboratory Medicine (검사의학전공) Journal Papers (저널논문_검사의학전공)
Green tea extract inhibits paraquat-induced pulmonary fibrosis by suppression of oxidative stress and endothelin-l expression
- Kim, Hak-Ryul; Park, Byung-Kyu; Oh, Yeon-Mok; Lee, Yun-Song; Lee, Dong-Soon; Kim, Hyun-Kuk; Kim, Joo-Young; Shim, Tae-Sun; Lee, Sang-Do
- Issue Date
- Springer Verlag
- Lung 184:287-295
- Camellia sinensis; Disease Models, Animal; Endothelin-1/biosynthesis/*genetics; Gene Expression/*drug effects; Herbicides/toxicity; Immunohistochemistry; Malondialdehyde/metabolism; Oxidative Stress/*drug effects; Paraquat/toxicity; Plant Extracts/*therapeutic use; Pulmonary Fibrosis/chemically induced/*drug therapy/metabolism; RNA/*genetics; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; Treatment Outcome
- Paraquat-induced pulmonary fibrosis involves two factors, direct injury by oxygen free radicals and indirect injury by inflammatory cells and fibroblasts. Endothelin-1 (ET-1) has been shown to act as a mediator of pulmonary fibrosis, and its formation increases during oxidative stress. We investigated whether green tea extract (GTE), which has antioxidant properties, inhibits paraquat-induced pulmonary fibrosis and whether ET-1 is involved in this process. Paraquat (0.3 mg/kg) was instilled into the right lungs of rats, following which the rats were either not further treated (Group P, n = 7), or they were administered 1% GTE mixed with feed (Group PG; n = 7) or the ET(A) receptor antagonist ZD2574 (10 mg/kg through gavage; Group PZ; n = 7) for two weeks. As control, we used rats instilled with saline (Group N; n = 6). Two weeks after paraquat instillation, we assayed the degree of pulmonary fibrosis by light microscopic morphometry and hydroxyproline content; lipid peroxidation as a marker of oxidative stresses by measurement of malondialdehyde (MDA); ET-1 by immunohistochemistry; and prepro-ET-1 mRNA expression by reverse transcription-polymerase chain reaction. Compared with Group N, significant pulmonary fibrosis was observed in Group P, accompanied by increases in MDA, ET-1, and prepro-ET-1 mRNA expression. Compared with Group P, Group PG showed significant decreases in pulmonary fibrosis, along with decreases in MDA, ET-1, and prepro-ET-1 mRNA expression. We also observed significant decreases in pulmonary fibrosis in Group PZ compared with Group P. These findings suggest that GTE inhibits paraquat-induced pulmonary fibrosis by suppression of oxidative stress and ET-1 expression.
- 0341-2040 (Print)
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