S-Space College of Engineering/Engineering Practice School (공과대학/대학원) Dept. of Electrical and Computer Engineering (전기·정보공학부) Theses (Ph.D. / Sc.D._전기·정보공학부)
Rapid drug susceptibility test of M. tuberculosis using single cell tracking method in agarose matrix
아가로즈 내에 단일 세포 추적 방법을 이용한 결핵균의 신속한 약제 감수성 검사 기법
- 공과대학 전기·컴퓨터공학부
- Issue Date
- 서울대학교 대학원
- Tuberculosis; Antibiotic resistant; Single cell tracking; Agarose; Drug susceptibility test
- 학위논문 (박사)-- 서울대학교 대학원 : 전기·컴퓨터공학부, 2015. 2. 권성훈.
- In this thesis, I describe a principle and system for rapid drug susceptibility test (DST) of M. tuberculosis (MTB). In the test system, single cell of MTB is immobilized in the agarose matrix and culture media with TB drugs are diffused into the agarose. The response of single cell is observed by microscopy and the images were transformed by image processing program for quantification. In case of common pathogen, antibiotic susceptibility test comparable with standard method was performed in 3 ~ 4 hours. Single cell morphological analysis is developed to handle the heterogeneous responses of bacteria in the various antimicrobial condition and the system derived an AST result satisfying the FDA standard for new AST systems. In the final, DST system for MTB was developed which can determine the DST of MDT in one week by single cell tracking method. Single cell tracking method in agarose matrix is validated as a solution for tackle the global health problem of tuberculosis and antibiotic resistance.