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(The) type III effector XopN of Xanthomonas oryzae pv. oryzae Targets OsVOZ2 and a putative thiamine synthase in rice
벼흰잎마름병균이 분비하는 Type III Effector XopN과 벼의 단백질 OsVOZ2, Putative Thiamine Synthase의 상호작용

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Authors
정훈
Advisor
황인규
Major
농업생명과학대학 농생명공학부
Issue Date
2014-02
Publisher
서울대학교 대학원
Keywords
Xanthomonas oryzae pv. oryzaexop genestype III secretion systemyeast two-hybridbimolecular fluorescence complementationpull-down assay
Description
학위논문 (박사)-- 서울대학교 대학원 : 농생명공학부, 2014. 2. 황인규.
Abstract
Bacterial leaf blight on rice, one of the most serious rice diseases, is caused by Xanthomonas oryzae pv. oryzae (Xoo). To determine roles of Xanthomonas outer proteins (Xop) in Xoo, I identified seven Xop homologs from the genome information of Xoo KXO85. The xopQKXO85, xopXKXO85, xopP2KXO85, xopP1KXO85, and xopNKXO85 were generated by EZ-Tn5 mutagenesis. Among the individual mutants, the xopP2KXO85, xopP1KXO85 and xopNKXO85 mutants exhibited significantly reduced virulence compared to the wild-type in the flag leaves of Dongjin. Interestingly, I did not see reduced virulence when the xopP2KXO85, xopP1KXO85 and xopNKXO85 mutants were inoculated into young leaves of the Dongjin. This indicated that Xop-mediated virulence is dependent on leaf stages in rice. To identify target proteins of XopNKXO85 in rice, I performed yeast two-hybrid screening. Two rice proteins, OsVOZ2 and putative thiamine synthase, appeared to interact with XopNKXO85 in yeast. This interaction was further confirmed by bimolecular fluorescence complementation and in vivo pull-down assays. OsVOZ2 is a hypothetical protein, and OsXNP is a putative thiamine synthase. To investigate roles of OsVOZ2 in interactions between rice and Xoo, I evaluated virulence of the wild-type and the xopNKXO85 mutant strain in the OsVOZ2 mutant rice generated by T-DNA insertion in Dongjin. Both the wild-type and the xopNKXO85 mutant were less virulent in the mutant rice than in the wild-type rice. Therefore, XopNKXO85 is a virulence factor that it plays an important role in the Xoo and rice interactions.
Language
English
URI
https://hdl.handle.net/10371/119458
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Agricultural Biotechnology (농생명공학부)Theses (Ph.D. / Sc.D._농생명공학부)
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