S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) Theses (Ph.D. / Sc.D._의학과)
Helicobacter pylori eradication down-regulates cellular inhibitor of apoptosis protein 2 in gastric carcinogenesis
위암에서 헬리코박터 파일로리 제균치료에 의한 cIAP2 발현의 하향 조절
- 의과대학 의학과
- Issue Date
- 서울대학교 대학원
- 학위논문 (박사)-- 서울대학교 대학원 : 의학과 내과학 전공, 2016. 8. 정현채.
- Background: To evaluate the expression of cellular inhibitor of apoptosis protein 2 (cIAP2) during gastric carcinogenesis after Helicobacter pylori (HP) infection and after HP eradication
Methods: We divided non-cancer patients into 4 groups according to the status of HP infection and atrophic gastritis (AG)/intestinal metaplasia (IM)
we compared cIAP2 mRNA expression among these 4 groups and patients with HP-positive early gastric cancer (EGC) by real-time PCR. We evaluated the expression of cIAP2 mRNA/protein by real-time PCR/immunohistochemistry, and the degree of apoptosis by TUNEL assay before and 12 months after endoscopic submucosal dissection (ESD) in HP-positive EGC patients irrespective of whether they received eradication therapy.
Results: The expression of cIAP2 mRNA was significantly higher in groups with HP(+) and AG/IM(+) and HP-positive EGC than in the control, HP(+) and AG/IM(-) groups (p < 0.005). In the HP eradication group, the expression of cIAP2 mRNA/protein significantly decreased (p = 0.006) and apoptosis increased at the 12-month follow-up after ESD. In the HP non-eradication group, the aforementioned changes were not found at the same follow-up period.
Conclusion: The expression of cIAP2 increased during gastric carcinogenesis after HP infection
HP eradication in the patients who underwent ESD for EGC reversed the over-expression of cIAP2 and suppressed cell apoptosis.