In vivo Inhibition of Porphyromonas gingivalis Growth and Prevention of Periodontitis With Quorum-Sensing Inhibitors
쿼럼 센싱 억제제를 이용한 Porphyromonas gingivalis 성장 억제 및 치주 질환 예방에 대한 in vivo 연구

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치의학대학원 치의과학과
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서울대학교 대학원
Biofilms models animal orphyromonas gingivalis Quorum sensing ribose
학위논문 (박사)-- 서울대학교 대학원 : 치의과학과, 2017. 2. 구기태.
Background: Autoinducer (AI)-2 has an important role in biofilm formation in the oral environment. Mature biofilms formed as a result of the cell to cell communication make it difficult to overcome periodontitis with the use of antibiotics. Previous in vitro studies suggest that quorum-sensing inhibitors (QSIs) interfere with AI-2. This study compared the QSI effects resulting from an oral inoculation of Porphyromonas gingivalis in an experimental animal model.

Methods: Forty-five male mice were divided into three groups (n = 15 each): 1) infection, 2) QSI, and 3) control. Infection and QSI groups received oral inoculation of P. gingivalis, whereas treatment with QSIs (furanone compound and D-ribose) was only performed in the QSIs group. The control group was a negative control not receiving manipulation. After 42 days, mice were sacrificed, and the distance from the alveolar bone crest (ABC) to the cemento-enamel junction (CEJ) was measured by microcomputed tomography. P. gingivalis DNA was quantified in the soft and hard tissues around the molar teeth by real-time polymerase chain reaction.

Results: Distance from ABC to CEJ was significantly increased in the P. gingivalis infection group compared with the control group (P = 0.02) and significantly decreased in the QSI group compared with the infection group (P = 0.02). The QSI group contained 31.64% of the bacterial DNA count of the infection group.

Conclusion: Use of QSIs in the mice infection model showed a reduction of bone breakdown and a decrease in the number of bacteria in vivo, suggesting that QSIs can be a new approach to prevention and treatment of periodontitis.
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dental Science(치의과학과)Theses (Ph.D. / Sc.D._치의과학과)
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