S-Space College of Natural Sciences (자연과학대학) Dept. of Biological Sciences (생명과학부) Theses (Master's Degree_생명과학부)
Effect of glucose in the growth of Dictyostelium discoideum : Dictyostelium discoideum의 생장에 미치는 포도당의 영향
- 자연과학대학 생명과학부
- Issue Date
- 서울대학교 대학원
- 학위논문 (석사)-- 서울대학교 대학원 : 생명과학부, 2015. 2. 강사욱.
- The glycation of proteins by glucose has been linked to the development of diabetic complications and other diseases. Early glycation is thought to involve the reaction of glucose with N- terminal chain amino groups to form Schiﬀs base and fructosamine adducts. The formation of the α-oxoaldehydes, glyoxal, methylglyoxal, in early glycation was investigated. Glucose degraded slowly to form glyoxal, methylglyoxal. Cells were cultured with high concentration of glucose in HL5 media showed cell growth inhibition and increase of the cell size and weight. And concentration of intracellular methylglyoxal measured drastically increased during cultured with high-dose glucose in media. This results supporting the high-dose glucose induced accumulation of methylglyoxal in vivo. To confirm this hypothesis, putrescine added under the same incubate conditions, and the result exhibited putrescine blocks cell growth inhibition caused by accumulation of methylglyoxal. These results suggest a possibility of accumulation of methylglyoxal from high-dose glucose.