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Rescue effects of small conductance Ca2+-activated K+ channel (SK4) activator on the Ca2+ overload induced death of head and neck cancer cells
칼슘과부하에 의한 두경부암세포 사멸현상에 대한 SK4 포타슘 이온통로 활성제의 세포사멸 방지효과

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Authors
윤명철
Advisor
김성준
Major
의과대학 의과학과
Issue Date
2016-02
Publisher
서울대학교 대학원
Keywords
편평 세포암IonomycinCa2+-activated K+ channel1-EBIO세포 증식
Description
학위논문 (석사)-- 서울대학교 대학원 : 의과학과 의과학전공, 2016. 2. 김성준.
Abstract
Ion channels in carcinoma and their roles in cell proliferation are drawing attention. Intracellular Ca2+ ([Ca2+]c)-dependent signaling affects the fate of cancer cells. K+ channels play critical roles in proliferation and apoptosis in many types of cancer cells. Small conductance Ca2+ activated K+ channel (SK4) is founded to be functional expressed in head and neck cancer cell carcinoma cells. The role of Ca2+-activated K+ channel (SK4) in head and neck squamous cell carcinoma (HNSCC) were investigated different cell lines
SNU-1076, OSC-19 and HN5 . Treatment with 1 μΜ ionomycin (Ca2+ ionophore) induced cell death in all of the three cell lines. Whole-cell patch clamp study suggested common expressions of Ca2+-activated Cl- channels (Ano-1) and Ca2+-activated nonselective cation channels (CAN). 1-EBIO, an activator of SK4, induced outward K+ current (ISK4) in SNU-1076 and OSC-19. In HN5, ISK4 was not observed or negligible. The 1-EBIO-induced current was abolished by TRAM-34, a selective SK4 blocker. Interestingly, the ionomycin-induced cell death was effectively prevented by 1-EBIO in SNU-1076 and OSC-19, and the rescue effect was annihilated by combined TRAM-34. Consistent with the lower level of ISK4, the rescue by 1-EBIO was least effective in HN5. The results demonstrate the role of SK4 in the fate of HNSCC under the Ca2+ overloaded condition. Pharmacological modulation of SK4 might provide an intriguing novel tool for the anti-cancer strategy in HNSCC.
Language
English
URI
https://hdl.handle.net/10371/133009
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biomedical Sciences (대학원 의과학과)Theses (Master's Degree_의과학과)
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