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Rescue effects of small conductance Ca2+-activated K+ channel (SK4) activator on the Ca2+ overload induced death of head and neck cancer cells
칼슘과부하에 의한 두경부암세포 사멸현상에 대한 SK4 포타슘 이온통로 활성제의 세포사멸 방지효과

DC Field Value Language
dc.contributor.advisor김성준-
dc.contributor.author윤명철-
dc.date.accessioned2017-07-19T10:41:13Z-
dc.date.available2017-07-19T10:41:13Z-
dc.date.issued2016-02-
dc.identifier.other000000132155-
dc.identifier.urihttps://hdl.handle.net/10371/133009-
dc.description학위논문 (석사)-- 서울대학교 대학원 : 의과학과 의과학전공, 2016. 2. 김성준.-
dc.description.abstractIon channels in carcinoma and their roles in cell proliferation are drawing attention. Intracellular Ca2+ ([Ca2+]c)-dependent signaling affects the fate of cancer cells. K+ channels play critical roles in proliferation and apoptosis in many types of cancer cells. Small conductance Ca2+ activated K+ channel (SK4) is founded to be functional expressed in head and neck cancer cell carcinoma cells. The role of Ca2+-activated K+ channel (SK4) in head and neck squamous cell carcinoma (HNSCC) were investigated different cell lines-
dc.description.abstractSNU-1076, OSC-19 and HN5 . Treatment with 1 μΜ ionomycin (Ca2+ ionophore) induced cell death in all of the three cell lines. Whole-cell patch clamp study suggested common expressions of Ca2+-activated Cl- channels (Ano-1) and Ca2+-activated nonselective cation channels (CAN). 1-EBIO, an activator of SK4, induced outward K+ current (ISK4) in SNU-1076 and OSC-19. In HN5, ISK4 was not observed or negligible. The 1-EBIO-induced current was abolished by TRAM-34, a selective SK4 blocker. Interestingly, the ionomycin-induced cell death was effectively prevented by 1-EBIO in SNU-1076 and OSC-19, and the rescue effect was annihilated by combined TRAM-34. Consistent with the lower level of ISK4, the rescue by 1-EBIO was least effective in HN5. The results demonstrate the role of SK4 in the fate of HNSCC under the Ca2+ overloaded condition. Pharmacological modulation of SK4 might provide an intriguing novel tool for the anti-cancer strategy in HNSCC.-
dc.description.tableofcontentsIntroduction 1

Material and Methods 4
1. Cell culture and proliferation assay 4
2. Electrophysiology 4
3. Solutions and Drugs 5
4. [Ca2+]i measurement 6
5. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) 6
6. Statistical Analysis 7

Results 8

Discussion 29

References 30

Abstract in Korean 37
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dc.formatapplication/pdf-
dc.format.extent3004555 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subject편평 세포암-
dc.subjectIonomycin-
dc.subjectCa2+-activated K+ channel-
dc.subject1-EBIO-
dc.subject세포 증식-
dc.subject.ddc610-
dc.titleRescue effects of small conductance Ca2+-activated K+ channel (SK4) activator on the Ca2+ overload induced death of head and neck cancer cells-
dc.title.alternative칼슘과부하에 의한 두경부암세포 사멸현상에 대한 SK4 포타슘 이온통로 활성제의 세포사멸 방지효과-
dc.typeThesis-
dc.contributor.AlternativeAuthorYIN MINGZHE-
dc.description.degreeMaster-
dc.citation.pages38-
dc.contributor.affiliation의과대학 의과학과-
dc.date.awarded2016-02-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biomedical Sciences (대학원 의과학과)Theses (Master's Degree_의과학과)
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