Publications
Detailed Information
Rescue effects of small conductance Ca2+-activated K+ channel (SK4) activator on the Ca2+ overload induced death of head and neck cancer cells : 칼슘과부하에 의한 두경부암세포 사멸현상에 대한 SK4 포타슘 이온통로 활성제의 세포사멸 방지효과
Cited 0 time in
Web of Science
Cited 0 time in Scopus
- Authors
- Advisor
- 김성준
- Major
- 의과대학 의과학과
- Issue Date
- 2016-02
- Publisher
- 서울대학교 대학원
- Keywords
- 편평 세포암 ; Ionomycin ; Ca2+-activated K+ channel ; 1-EBIO ; 세포 증식
- Description
- 학위논문 (석사)-- 서울대학교 대학원 : 의과학과 의과학전공, 2016. 2. 김성준.
- Abstract
- Ion channels in carcinoma and their roles in cell proliferation are drawing attention. Intracellular Ca2+ ([Ca2+]c)-dependent signaling affects the fate of cancer cells. K+ channels play critical roles in proliferation and apoptosis in many types of cancer cells. Small conductance Ca2+ activated K+ channel (SK4) is founded to be functional expressed in head and neck cancer cell carcinoma cells. The role of Ca2+-activated K+ channel (SK4) in head and neck squamous cell carcinoma (HNSCC) were investigated different cell lines
SNU-1076, OSC-19 and HN5 . Treatment with 1 μΜ ionomycin (Ca2+ ionophore) induced cell death in all of the three cell lines. Whole-cell patch clamp study suggested common expressions of Ca2+-activated Cl- channels (Ano-1) and Ca2+-activated nonselective cation channels (CAN). 1-EBIO, an activator of SK4, induced outward K+ current (ISK4) in SNU-1076 and OSC-19. In HN5, ISK4 was not observed or negligible. The 1-EBIO-induced current was abolished by TRAM-34, a selective SK4 blocker. Interestingly, the ionomycin-induced cell death was effectively prevented by 1-EBIO in SNU-1076 and OSC-19, and the rescue effect was annihilated by combined TRAM-34. Consistent with the lower level of ISK4, the rescue by 1-EBIO was least effective in HN5. The results demonstrate the role of SK4 in the fate of HNSCC under the Ca2+ overloaded condition. Pharmacological modulation of SK4 might provide an intriguing novel tool for the anti-cancer strategy in HNSCC.
- Language
- English
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.