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Chemical Constituents of the Seeds of Aesculus turbinata and Their PEDV Inhibitory Activity
칠엽수 열매로부터 얻은 화학성분과 PEDV 저해 활성 확인

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Authors
김지원
Advisor
오원근
Major
약학대학 약학과
Issue Date
2017-02
Publisher
서울대학교 대학원
Keywords
Aesculus turbinata Bl.Sapindaceaeescincytotoxicityhydrolysisoleanane-type triterpenoidsPEDV.
Description
학위논문 (석사)-- 서울대학교 대학원 : 약학대학 약학과(생약학전공), 2017. 2. 오원근.
Abstract
Porcine epidemic diarrhea virus (PEDV) causes severe diarrhea and high fatality of piglets, influencing the swine industry. Japanese horse chestnut (seed of Aesculus turbinata) contains many saponin mixtures, called escins, and has been used for a long time as a traditional medicinal plant. Structure-activity relationship (SAR) studies on escins have revealed that acylations at C-21 and C-22 with angeloyl or tigoloyl groups were important for their cytotoxic effects. However, the strong cytotoxicity of escins makes them hard to utilize for other diseases and to develop as nutraceuticals. In this research, we investigated whether escin derivatives 1-7 (including new compounds 2, 3, 5 and 6), without the angeloyl or tigloyl groups and with modified glycosidic linkages by hydrolysis, have PEDV inhibitory effects with less cytotoxicity than the major compounds 8-10 isolated from the extract. Compounds 1-7 had no cytotoxicity at 20 μM on VERO cells, while compounds 8-10 showed strong cytotoxicity at similar concentrations, and also had moderate inhibitory activities on PEDV. Our results suggest that escin derivatives, which were prepared by two-step reactions, showed strong inhibitory activities on PEDV replication with less cytotoxicity. These studies proposed a method to utilize Japanese horse chestnut for treating PEDV with less cytotoxic effects, and to increase the diversity of its bioactive compounds.
Language
English
URI
https://hdl.handle.net/10371/133660
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College of Pharmacy (약학대학)Dept. of Pharmacy (약학과)Theses (Master's Degree_약학과)
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