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Effect of genistein supplementation on methionine-choline deficient diet-induced nonalcoholic fatty liver disease development in db/db mice : 제니스테인의 보충섭취가 db/db 마우스에서 메티오닌-콜린 결핍 식이에 의해 유도된 비알코올성 지방간질환에 미치는 영향
DC Field | Value | Language |
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dc.contributor.advisor | 권영혜 | - |
dc.contributor.author | 유나영 | - |
dc.date.accessioned | 2017-07-19T11:56:41Z | - |
dc.date.available | 2017-07-19T11:56:41Z | - |
dc.date.issued | 2014-08 | - |
dc.identifier.other | 000000021760 | - |
dc.identifier.uri | https://hdl.handle.net/10371/133939 | - |
dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 식품영양학과, 2014. 8. 권영혜. | - |
dc.description.abstract | Nonalcoholic fatty liver disease (NAFLD) occurs in a wide spectrum from simple steatosis to steatohepatitis, fibrosis and rarely, progression to cirrhosis. When db/db mice are fed a methionine-choline deficient (MCD) diet, significant liver fibrosis is observed as compared to other animal models. Previous studies reported that genistein has been shown to attenuate non-alcoholic steatohepatitis (NASH) induced in obese and type 2 diabetic animal models. However, there have been no studies of effect of genistein on NASH using animal model which has weight loss, anti-NASH effect of genistein is unclear. Therefore, we investigated the effect of genistein on the development of NASH induced by an MCD diet in db/db mice. Male C57BL/KsJdb/db mice were fed a control diet or MCD diet for 6 weeks. To investigate the effect of genistein, the additional group fed an MCD diet with 0.05% genistein. MCD diet-fed mice exhibited a significantly lower body and liver weight. And serum glucose, insulin, cholesterol and alanine aminotransferase levels were also decreased by MCD diet. MCD diet develops a higher degree of steatohepatitis corresponding with an increased oxidative stress, endoplasmic reticulum stress, hepatic steatosis, inflammation, stellate cell activation and mild fibrosis. Genistein reduced serum insulin levels, but we did not observe any inhibitory effect against hepatic steatosis by genistein supplementation. In contrast, hepatic oxidative stress and endoplasmic reticulum stress, and AMP-dependent kinase inactivation were alleviated by genistein. Genistein also down-regulated the augmented mRNA expression of genes associated with inflammatory cytokines or chemokines (tumor necrosis factor α, monocyte chemoattractant protein 1, toll-like receptor 4 and interleukin 1β) and fibrosis (transforming growth factor β1, procollagen type Ⅰ and tissue inhibitors of metalloproteinase 1). In conclusion, these results suggest the genistein alleviated MCD diet-mediated NASH development by suppressing lipid peroxidation, inflammation and even liver fibrosis in db/db mice. | - |
dc.description.tableofcontents | Abstract ⅰ
Contents ⅲ List of Figures ⅴ List of Tables ⅵ List of Abbreviations ⅶ Introduction 1 Materials and Methods 14 Animals and diets 14 Body weight and fasting blood glucose 16 Blood and tissue collection 16 Serum biochemical analyses 16 Hepatic biochemical analyses 17 Liver histology examination 18 Total protein extraction and immunoblotting 18 Total RNA extraction and semi-quantitative PCR analyses 20 SYBR green real-time PCR analyses 20 Statistical analysis 21 Results 23 Effect of genistein on changes of body weight and fasting blood glucose during the experimental period 23 Effect of genistein on final body weight, organ weight and food consumption 25 Effect of genistein on serum biochemical analyses 27 Effect of genistein on hepatic lipid profiles and liver histology 29 Effect of genistein on oxidative stress 32 Effect of genistein on ER stress 34 Effect of genistein on inflammatory response 37 Effect of genistein on liver fibrosis 41 Discussion 44 References 49 국문초록 59 | - |
dc.format | application/pdf | - |
dc.format.extent | 1111149 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | db/db mouse | - |
dc.subject | fibrosis | - |
dc.subject | genistein | - |
dc.subject | methionine-choline deficient diet | - |
dc.subject | non-alcoholic steatohepatitis | - |
dc.subject.ddc | 641 | - |
dc.title | Effect of genistein supplementation on methionine-choline deficient diet-induced nonalcoholic fatty liver disease development in db/db mice | - |
dc.title.alternative | 제니스테인의 보충섭취가 db/db 마우스에서 메티오닌-콜린 결핍 식이에 의해 유도된 비알코올성 지방간질환에 미치는 영향 | - |
dc.type | Thesis | - |
dc.description.degree | Master | - |
dc.citation.pages | ⅷ, 61 | - |
dc.contributor.affiliation | 생활과학대학 식품영양학과 | - |
dc.date.awarded | 2014-08 | - |
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