Preparation of fucose-containing monosaccharides from Undaria pinnatifida sporophyll and synthesis of fucosylated oligossacharide using Bifidobacterium longum RD47

Abstract

Human milk oligosaccharides (HMOs) are mostly fucosylated at their non-reducing termini. HMOs are known to function as a prebiotic for bifidobacteria. L-fucose is major component of HMOs but very costly. Fucoidan is a group of marine sulfated polysaccharide of the cell-wall matrix of brown algae, containing large proportions of L-fucose. In this research, L-fucose containing monosaccharides (FCMs) were obtained from fucoidan. For the experiment, fucoidan was extracted from U. pinnatifida sporophyll using 0.03 M HCl. Crude fucoidan was purified by ion-exchange chromatography and hydrolyzed to obtain FCMs. The analysis of monosaccharides composition was performed by Bio-LC. FCMs were applied to gel permeation column and used as a substrate to synthesize FO using the crude enzyme extracts from Bifidobacterium longum RD47. The synthesized FO was detected by thin layer chromatography. In the application aspect, the FO may provide a new constituent for cost-effective prebiotic food ingredient.