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Effect of Novel Propionyl-fructooligosaccharides on Growth of Intestinal Bacteria : 신규 프로피오닐 프락토올리고당이 장내 균 성장에 미치는 영향
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 지근억 | - |
| dc.contributor.author | 이승은 | - |
| dc.date.accessioned | 2017-07-19T11:58:41Z | - |
| dc.date.available | 2017-07-19T11:58:41Z | - |
| dc.date.issued | 2017-02 | - |
| dc.identifier.other | 000000140713 | - |
| dc.identifier.uri | https://hdl.handle.net/10371/133982 | - |
| dc.description | 학위논문 (석사)-- 서울대학교 대학원 : 식품영양학과, 2017. 2. 지근억. | - |
| dc.description.abstract | Numerous studies reported that ingestion of fructooligosaccharides (FOS) can promote the growth of Bifidobacterium in large intestine. Therefore, FOS is currently used as prebiotics. Propionic acid (PA) has an inhibitory effect on the growth of pathogenic molds and bacteria. Propionates such as sodium propionate and calcium propionate are used as preservatives for food. In this study, the effect of novel propionyl-fructooligosaccharides (P-FOS) on the growth of various intestinal bacteria was assessed. According to the structural analyses using FT-IR, MALDI-TOF MS, LC-ESI-MS, and LC-ESI-MS/MS, the major components of P-FOS used in this study contained FOS with 1-3 propionyl groups attached. P-FOS promoted the growth of the most experimental Bifidobacterium and some of the other lactic acid bacteria. In contrast to FOS, P-FOS showed no growth promotion or slight suppression against most of the non-probiotic bacteria. The novel P-FOS is expected to be useful for the improvement of human intestinal microflora. | - |
| dc.description.tableofcontents | 1. Introduction 1
2. Materials and methods 4 2.1. Materials 4 2.1.1. Source of P-FOS 4 2.1.2. The bacterial strains and culture condition 4 2.1.3. Chemicals and reagents 7 2.1.4. The media and carbohydrate sources for bacterial growth test 7 2.2. Purification and preparation of P-FOS 7 2.3. Structural analysis of P-FOS 8 2.3.1. Determination of the linkages in P-FOS by FT-IR 8 2.3.2. Mass analysis by MALDI-TOF MS and LC-ESI-MS 8 2.4. Effect of glucose, FOS, and P-FOS on growth of intestinal bacteria 9 3. Results and Discussion 11 3.1. Structural analysis of P-FOS 11 3.1.1. Investigation of linkages in P-FOS using FT-IR 11 3.1.2. Mass spectra analysis of FOS by MALDI-TOF MS and LC-ESI-MS 14 3.1.3. Mass spectra analysis of P-FOS by MALDI-TOF MS, LC-ESI-MS, and LC-ESI-MS/MS 16 3.2. Growth of various bacteria in the presence of P-FOS, FOS, and glucose 20 4. Conclusion 28 References 29 Abstract in Korean 34 | - |
| dc.format | application/pdf | - |
| dc.format.extent | 1217385 bytes | - |
| dc.format.medium | application/pdf | - |
| dc.language.iso | en | - |
| dc.publisher | 서울대학교 대학원 | - |
| dc.subject | fructooligosaccharides | - |
| dc.subject | bacterial growth | - |
| dc.subject | structural analysis of oligosaccharides | - |
| dc.subject.ddc | 641 | - |
| dc.title | Effect of Novel Propionyl-fructooligosaccharides on Growth of Intestinal Bacteria | - |
| dc.title.alternative | 신규 프로피오닐 프락토올리고당이 장내 균 성장에 미치는 영향 | - |
| dc.type | Thesis | - |
| dc.contributor.AlternativeAuthor | Lee, Seung-Eun | - |
| dc.description.degree | Master | - |
| dc.citation.pages | vii, 34 | - |
| dc.contributor.affiliation | 생활과학대학 식품영양학과 | - |
| dc.date.awarded | 2017-02 | - |
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