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Simvastatin protects ischemic spinal cord injury from cell death and cytotoxicity through decreasing oxidative stress : in vitro primary cultured rat spinal cord model under oxygen and glucose deprivation-reoxygenation conditions : 척수 허혈-재관류 손상 시 심바스타틴이 산화스트레스 감소를 통해 세포 사멸 및 세포 독성에 미치는 신경 보호 효과: 산소 및 포도당 결핍-재산소화 생체 외 1차 배양 흰쥐의 척수 모델
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 한성희 | - |
dc.contributor.author | 손혜민 | - |
dc.date.accessioned | 2017-10-27T17:06:00Z | - |
dc.date.available | 2017-10-27T17:06:00Z | - |
dc.date.issued | 2017-08 | - |
dc.identifier.other | 000000145528 | - |
dc.identifier.uri | https://hdl.handle.net/10371/137078 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 의과대학 의학과, 2017. 8. 한성희. | - |
dc.description.abstract | Background: Ischemia and the following reperfusion damage are critical mechanisms of spinal cord injury. Statins have been reported to decrease ischemia-reperfusion injury in many organs including spinal cord. Anti-oxidative effect is one of the main protective mechanisms of statin against neuronal death and cytotoxicity. We hypothesized that statins anti-oxidative property would yield neuroprotective effects on spinal cord ischemia-reperfusion injury
Methods: Primary cultured spinal cord motor neurons were isolated from Sprague-Dawley rat fetuses. Ischemia–reperfusion injury model was induced by 60 min of oxygen and glucose deprivation (OGD) and 24 h of reoxygenation. Healthy and OGD cells were treated with simvastatin at concentrations of 0.1, 1, and 10 μM for 24 h. Cell viability was assessed using WST-8, cytotoxicity with LDH, and production of free radicals with DCFDA. Results: OGD reduced neuronal viability compared to normoxic control by 35.3% | - |
dc.description.abstract | however 0.1-10 μM of simvastatin treatment following OGD improved cell survival. OGD increased LDH release up to 214% | - |
dc.description.abstract | however, simvastatin treatment attenuated its cytotoxicity at concentrations of 0.1-10 μM (p < 0.001 and p = 0.001). Simvastatin also reduced deteriorated morphological changes of motor neurons following OGD. Oxidative stress was reduced by simvastatin (0.1-10 μM) compared to untreated cells exposed to OGD (p < 0.001).
Conclusions: Simvastatin effectively reduced spinal cord neuronal death and cytotoxicity against ischemia-reperfusion injury, probably via modification of oxidative stress. | - |
dc.description.tableofcontents | 서 론 1
대상 및 방법 3 결 과 8 고 찰 15 참고문헌 19 국문초록 25 | - |
dc.format | application/pdf | - |
dc.format.extent | 1037634 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | 산소-포도당 결핍 | - |
dc.subject | 산화 스트레스 | - |
dc.subject | 신경 보호 | - |
dc.subject | 심바스타틴 | - |
dc.subject | 척수 손상 | - |
dc.subject | 허혈-재관류 손상 | - |
dc.subject.ddc | 610 | - |
dc.title | Simvastatin protects ischemic spinal cord injury from cell death and cytotoxicity through decreasing oxidative stress : in vitro primary cultured rat spinal cord model under oxygen and glucose deprivation-reoxygenation conditions | - |
dc.title.alternative | 척수 허혈-재관류 손상 시 심바스타틴이 산화스트레스 감소를 통해 세포 사멸 및 세포 독성에 미치는 신경 보호 효과: 산소 및 포도당 결핍-재산소화 생체 외 1차 배양 흰쥐의 척수 모델 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Sohn, Hye-Min | - |
dc.description.degree | Doctor | - |
dc.contributor.affiliation | 의과대학 의학과 | - |
dc.date.awarded | 2017-08 | - |
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