Publications

Detailed Information

Physicochemical Characteristics and Anti-Oxidant Activities of Farm-Cultivated and Mountain-Cultivated Ginseng Seeds : 인삼 및 산양삼 씨앗의 이화학적 특성 및 항산화능

DC Field Value Language
dc.contributor.advisor황금택-
dc.contributor.author김유정-
dc.date.accessioned2017-10-31T08:16:27Z-
dc.date.available2017-10-31T08:16:27Z-
dc.date.issued2017-08-
dc.identifier.other000000145216-
dc.identifier.urihttps://hdl.handle.net/10371/137894-
dc.description학위논문 (석사)-- 서울대학교 대학원 생활과학대학 식품영양학과, 2017. 8. 황금택.-
dc.description.abstract인삼(Panax ginseng Meyer)은 항노화, 항염증, 항산화, 항종양과 같은 생리 활성을 지닌 약용 식물로 널리 사용되어 왔다. 재배 조건이 인삼의 씨앗, 씨앗 기름, 잎, 뿌리의 화학적 특성에 미치는 영향에 대한 연구들이 보고 되었으나, 인삼 및 산양삼의 씨앗 기름과 씨앗 탈지박에 대한 연구는 부족하다. 따라서 본 연구에서는 인삼 및 산양삼 씨앗 기름의 이화학적 특성을 분석하고 탈지한 씨박의 항산화 물질과 항산화능을 측정하였다. 인삼 및 산양삼 씨앗의 조지방, 조단백질, 조회분의 함량은 각각 17.9-22.1, 11.5-15.2, 1.4-1.7%(dry basis)이었다. 인삼 및 산양삼 씨앗 기름의 95% 이상이 불포화 지방산이었고, 주요 지방산은 올레산(77.9-78.5%)과 리놀레산(16.6-17.4%)이었다. 산양삼 씨앗 기름은 인삼 씨앗 기름보다 Hunter b 값이 유의적으로 높았고, carotenoid (4.3 μg β-carotene equivalent/g oil), β/γ-tocotrienol (3.7 mg/100 g oil), δ-tocotrienol (0.2 mg/100 g oil), squalene (405.3 mg/100 g oil) 함량이 유의적으로 더 많았다(p<0.05). 씨앗 기름의 주요 phytosterol은 β-sitosterol (48.1-83.6 mg/100 g oil)이었고 산양삼 씨앗 기름에 가장 많았다. 인삼 및 산양삼 탈지 씨박의 조사포닌 함량은 7.9-8.5 mg/g dried meal이었다. 산양삼의 탈지한 씨박 추출물은 인삼의 탈지한 씨박 추출물보다 총 폴리페놀(28.1 mg gallic acid equivalent/g dried extracts)과 플라보노이드(5.5 mg quercetin equivalent/g dried extracts)가 유의적으로 많았다(p<0.05). 또한, 산양삼의 탈지한 씨박 추출물의 DPPH와 ABTS 라디칼 소거능, FRAP 값이 가장 높았다 (p<0.05). 결론적으로 재배 환경은 인삼 및 산양삼 씨앗 기름의 이화학적 특성 및 탈지한 씨박의 항산화물질과 항산화능에 영향을 미칠 수 있을 것으로 판단한다.-
dc.description.abstractBackground: Ginseng (Panax ginseng Meyer) has been well known for a medicinal plant. Effect of cultivations on chemical characteristics of ginseng plants has been studied. However, seed oils and defatted seed meals from farm-cultivated (FG) and mountain-cultivated ginsengs (MG) have not been studied.
Methods: Fatty acid compositions, carotenoids, tocols, squalene, and phytosterols of seed oils from two FG and one MG were analyzed. Crude saponin contents, phenolic and flavonoid contents, and anti-oxidant activities of defatted seed meals from the FG and MG were also determined.
Results: Crude lipids, crude proteins, and ash in the seeds were 17.9-22.1% (dry basis), 11.5-15.2%, and 1.4-1.7%, respectively. The major fatty acid in the seed oils was oleic acid (77.9-78.5%), followed by linoleic acid (16.6-17.4%). The seed oils from MG had higher Hunter b value, carotenoids (4.3 μg β-carotene equivalent/g oil), β/γ-tocotrienol (3.7 mg/100 g oil), and δ-tocotrienol (0.2 mg/100 g oil), and lower α-tocotrienol (20.8 mg/100 g oil) than the others. The MG seed oils had also more squalene (405.3 mg/100 g oil) than the others. The major phytosterol in the oils was β-sitosterol (48.1-83.6 mg/100 g oil) with MG the highest. Crude saponins in the defatted ginseng seed meals were 7.9-8.5 mg/g dried meal. Defatted seed meal extracts from MG had more total phenolics (28.1 mg gallic acid equivalent/g dried extracts) and flavonoids (5.5 mg quercetin equivalent/g dried extracts), and higher DPPH or ABTS radical scavenging activities and ferric reducing anti-oxidant power than the others.
Conclusion: Physicochemical characteristics of ginseng seed oil and anti-oxidant activities of defatted ginseng seed meal might be affected by cultivation environment.
-
dc.description.tableofcontentsABSTRACT I
CONTENTS III
LIST OF TABLES V
LIST OF FIGURES VI
INTRODUCTION 1
MATERIALS AND METHODS 3
1. Materials and reagent 3
2. Preparation of samples 4
3. Analysis of proximate composition of ginseng seeds 4
4. Analysis of fatty acid composition of ginseng seed oils 4
5. Analysis of color of ginseng seed oils 5
6. Analysis of carotenoids in ginseng seed oils 5
7. Analysis of tocols (tocopherols and tocotrienols) in ginseng seed oils 5
8. Preparation of unsaponifiable fraction of ginseng seed oils 6
9. Analysis of squalene and phytosterols in ginseng seed oils 7
10. Determination of crude saponin in defatted ginseng seed meals 8
11. Determination of total phenolics and flavonoids in defatted ginseng seed meal extracts 8
12. Anti-oxidant activities of defatted ginseng seed meal extracts 9
13. Statistical analysis 10
RESULTS AND DISCUSSION 11
1. Proximate composition of ginseng seeds 11
2. Yields of oils and defatted meal extracts from ginseng seeds 11
3. Fatty acid composition of ginseng seed oils 11
4. Color and carotenoid contents of ginseng seed oils 14
5. Tocols in ginseng seed oils 14
6. Squalene and phytosterols in ginseng seed oils 17
7. Crude saponin in defatted ginseng seed meals 22
8. Total phenolics and flavonoids in defatted ginseng seed meal extracts 22
9. Anti-oxidant activities of defatted ginseng seed meal extracts 24
CONCLUSION 26
REFERENCES 27
국문초록 33
-
dc.formatapplication/pdf-
dc.format.extent1013817 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectAnti-oxidant-
dc.subjectDefatted ginseng seed meal-
dc.subjectFarm-cultivated ginseng-
dc.subjectGinseng seed oil-
dc.subjectMountain-cultivated ginseng-
dc.subject.ddc641-
dc.titlePhysicochemical Characteristics and Anti-Oxidant Activities of Farm-Cultivated and Mountain-Cultivated Ginseng Seeds-
dc.title.alternative인삼 및 산양삼 씨앗의 이화학적 특성 및 항산화능-
dc.typeThesis-
dc.contributor.AlternativeAuthorKim Yu Jeong-
dc.description.degreeMaster-
dc.contributor.affiliation생활과학대학 식품영양학과-
dc.date.awarded2017-08-
Appears in Collections:
Files in This Item:

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share