S-Space College of Natural Sciences (자연과학대학) Dept. of Biological Sciences (생명과학부) Theses (Master's Degree_생명과학부)
Studies on the transcriptional regulation of Lefty1 during neuronal differentiation of P19 embryonic carcinoma cells
P19 배아줄기암세포가 신경세포 분화하는 과정에서 Lefty1 유전자의 전사 조절 기전 연구
- Eunsong Choi
- 자연과학대학 생명과학부
- Issue Date
- 서울대학교 대학원
- 학위논문 (석사)-- 서울대학교 대학원 자연과학대학 생명과학부, 2017. 8. 이건수.
- Stem cell division should be tightly controlled for a proper balance between self-renewal and differentiation. It is recently known that some types of stem cells have to undergo minimal rounds of cell division in order to turn on expression of a set of genes for differentiation. Here, I searched for genes of which the expression is controlled in a cell-division-dependent manner during neuronal differentiation of P19 embryonic carcinoma cells. I identified three candidate genes, Lefty1, Msi2, and Prtg of which the expressions were upregulated during the retinoic acid-induced neurogenesis in P19 cells. Their expressions were induced by RA in a delayed manner, and such induction was significantly dampened by cell cycle blocking drugs such as thymidine and RO3306. I defined a domain within the Lefty1 promoter sequence that is responsible for cell-division-dependent activation. These results provide a clue on mechanisms how gene expression is controlled by cell division for neuronal differentiation.