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Synergistic production of 20(S)-protopanaxadiol from protopanaxadiol-type ginsenosides by β-glycosidases from Dictyoglomus turgidum and Caldicellulosiruptor bescii
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Ji-Hyeon | - |
dc.contributor.author | Seo, Min-Ju | - |
dc.contributor.author | Shin, Kyung-Chul | - |
dc.contributor.author | Lee, Ki Won | - |
dc.contributor.author | Oh, Deok-Kun | - |
dc.date.accessioned | 2017-12-18T01:08:37Z | - |
dc.date.available | 2017-12-18T10:09:45Z | - |
dc.date.issued | 2017-12-14 | - |
dc.identifier.citation | AMB Express, 7(1):219 | ko_KR |
dc.identifier.issn | 2191-0855 | - |
dc.identifier.uri | https://hdl.handle.net/10371/138454 | - |
dc.description.abstract | Abstract
20(S)-Protopanaxadiol (APPD) has potential uses in the pharmaceutical, cosmetic, and food industries because of its anti-stress, anti-fatigue, anti-cancer, anti-inflammatory, and anti-wrinkle properties. However, APPD production is difficult because β-glycosidases that convert the protopanaxadiol (PPD)-type ginsenoside compound K to APPD are rare. β-Glycosidase from Dictyoglomus turgidum (DT-bgl) has the highest specific activity for converting compound K to APPD, but exhibits no activity towards the α-l-arabinopyranoside moiety in compound Y. Therefore, β-glycosidase from Caldicellulosiruptor bescii (CB-bgl), which has a strong α-l-arabinopyranosidase activity, was used along with DT-bgl. The volumetric and specific productivities of the two-enzyme system for APPD using ginseng root extract were 38.4- and 38.7-fold higher, respectively, than those of β-glycosidase from Pyrococcus furiosus, which had the highest volumetric productivity previously reported, at the same enzyme and substrateconcentrations. Thus, DT-bgl combined with CB-bgl completely converted PPD-type ginsenosides to APPD with the highest volumetric and specific productivities reported thus far. | ko_KR |
dc.description.sponsorship | This work was supported by the Nano-Convergence Foundation (http://www.nanotech2020.org) funded by the Ministry of Science and ICT (MSIT) and the Ministry of Trade, Industry and Energy (MOTIE), Republic of Korea (Project Number: R201602410). | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | Springer Open | ko_KR |
dc.subject | α-l-Arabinopyranosidase activity | ko_KR |
dc.subject | 20(S)-Protopanaxadiol | ko_KR |
dc.subject | β-Glycosidase | ko_KR |
dc.subject | Dictyoglomus turgidum | ko_KR |
dc.subject | Caldicellulosiruptor bescii | ko_KR |
dc.title | Synergistic production of 20(S)-protopanaxadiol from protopanaxadiol-type ginsenosides by β-glycosidases from Dictyoglomus turgidum and Caldicellulosiruptor bescii | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 최지현 | - |
dc.contributor.AlternativeAuthor | 서민주 | - |
dc.contributor.AlternativeAuthor | 신경철 | - |
dc.contributor.AlternativeAuthor | 이기원 | - |
dc.contributor.AlternativeAuthor | 오덕건 | - |
dc.identifier.doi | 10.1186/s13568-017-0524-9 | - |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2017-12-17T04:55:51Z | - |
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