S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dental Research Institute (치학연구소) Journal Papers (저널논문_치학연구소)
Complex Intratissue Microbiota Forms Biofilms in Periodontal Lesions
- Baek, K.; Ji, S.; Choi, Y.
- Issue Date
- SAGE PUBLICATIONS INC
- Journal of Dental Research, Vol.97 No.2, pp. 192-200
- Complex Intratissue Microbiota Forms Biofilms in Periodontal Lesions; 의약학; periodontitis; gingiva; microbial ecology; bacteria; Fusobacterium nucleatum
- Periodontitis is caused by dysbiotic subgingival bacterial communities that may lead to increased bacterial invasion into gingival tissues. Although shifts in community structures associated with transition from health to periodontitis have been well characterized, the nature of bacteria present within the gingival tissue of periodontal lesions is not known. To characterize microbiota within tissues of periodontal lesions and compare them with plaque microbiota, gingival tissues and subgingival plaques were obtained from 7 patients with chronic periodontitis. A sequencing analysis of the 16S rRNA gene revealed that species richness and diversity were not significantly different between the 2 groups. However, intersubject variability of intratissue communities was smaller than that of plaque communities. In addition, when compared with the plaque communities, intratissue communities were characterized by decreased abundance of Firmicutes and increased abundance of Fusobacteria and Chloroflexi. In particular, Fusobacterium nucleatum and Porphyromonas gingivalis were highly enriched within the tissue, composing 15% to 40% of the total bacteria. Furthermore, biofilms, as visualized by alcian blue staining and atomic force microscopy, were observed within the tissue where the degradation of connective tissue fibers was prominent. In conclusion, very complex bacterial communities exist in the form of biofilms within the gingival tissue of periodontal lesions, which potentially serve as a reservoir for persistent infection. This novel finding may prompt new research on therapeutic strategies to treat periodontitis.
- Files in This Item: There are no files associated with this item.