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Complex Intratissue Microbiota Forms Biofilms in Periodontal Lesions

Cited 21 time in Web of Science Cited 17 time in Scopus
Authors
Baek, K.; Ji, S.; Choi, Y.
Issue Date
2017-10
Publisher
SAGE PUBLICATIONS INC
Citation
Journal of Dental Research, Vol.97 No.2, pp. 192-200
Keywords
Complex Intratissue Microbiota Forms Biofilms in Periodontal Lesions의약학periodontitisgingivamicrobial ecologybacteriaFusobacterium nucleatum
Abstract
Periodontitis is caused by dysbiotic subgingival bacterial communities that may lead to increased bacterial invasion into gingival tissues. Although shifts in community structures associated with transition from health to periodontitis have been well characterized, the nature of bacteria present within the gingival tissue of periodontal lesions is not known. To characterize microbiota within tissues of periodontal lesions and compare them with plaque microbiota, gingival tissues and subgingival plaques were obtained from 7 patients with chronic periodontitis. A sequencing analysis of the 16S rRNA gene revealed that species richness and diversity were not significantly different between the 2 groups. However, intersubject variability of intratissue communities was smaller than that of plaque communities. In addition, when compared with the plaque communities, intratissue communities were characterized by decreased abundance of Firmicutes and increased abundance of Fusobacteria and Chloroflexi. In particular, Fusobacterium nucleatum and Porphyromonas gingivalis were highly enriched within the tissue, composing 15% to 40% of the total bacteria. Furthermore, biofilms, as visualized by alcian blue staining and atomic force microscopy, were observed within the tissue where the degradation of connective tissue fibers was prominent. In conclusion, very complex bacterial communities exist in the form of biofilms within the gingival tissue of periodontal lesions, which potentially serve as a reservoir for persistent infection. This novel finding may prompt new research on therapeutic strategies to treat periodontitis.
ISSN
0022-0345
Language
English
URI
https://hdl.handle.net/10371/139296
DOI
https://doi.org/10.1177/0022034517732754
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dental Research Institute (치학연구소)Journal Papers (저널논문_치학연구소)
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