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Superresolution fluorescence microscopy for 3D reconstruction of thick samples

Cited 6 time in Web of Science Cited 8 time in Scopus
Authors
Park, Sangjun; Kang, Wooyoung; Kwon, Yeong-Dae; Shim, Jaehoon; Kim, Siyong; Kaang, Bong-Kiun; Hohng, Sungchul
Issue Date
2018-03-15
Publisher
BioMed Central
Citation
Molecular Brain, 11(1):17
Keywords
Line-scan confocal microscopyDNA-PAINTSuperresolution microscopySingle-molecule localizationmicroscopyThree-dimensional reconstruction
Abstract
Abstract
Three-dimensional (3D) reconstruction of thick samples using superresolution fluorescence microscopy remains challenging due to high level of background noise and fast photobleaching of fluorescence probes. We develop superresolution fluorescence microscopy that can reconstruct 3D structures of thick samples with both high localization accuracy and no photobleaching problem. The background noise is reduced by optically sectioning the sample using line-scan confocal microscopy, and the photobleaching problem is overcome by using the DNA-PAINT (Point Accumulation for Imaging in Nanoscale Topography). As demonstrations, we take 3D superresolution images of microtubules of a whole cell, and two-color 3D images of microtubules and mitochondria. We also present superresolution images of chemical synapse of a mouse brain section at different z-positions ranging from 0 μm to 100 μm.
ISSN
1756-6606
Language
English
URI
http://hdl.handle.net/10371/139695
DOI
https://doi.org/10.1186/s13041-018-0361-z
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College of Natural Sciences (자연과학대학)Dept. of Physics and Astronomy (물리·천문학부)Physics (물리학전공)Journal Papers (저널논문_물리학전공)
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